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Cis-regulation of the amino acid transporter genes ZmAAP2 and ZmLHT1 by ZmPHR1 transcription factors in maize ear under phosphate limitation.
Wang, Ruifeng; Zhong, Yanting; Liu, Xiaoting; Zhao, Cheng; Zhao, Jianyu; Li, Mengfei; Ul Hassan, Mahmood; Yang, Bo; Li, Dongdong; Liu, Renyi; Li, Xuexian.
Afiliación
  • Wang R; The Key Laboratory of Plant-Soil Interactions, MOE, Department of Plant Nutrition, China Agricultural University, Beijing, China.
  • Zhong Y; The Key Laboratory of Plant-Soil Interactions, MOE, Department of Plant Nutrition, China Agricultural University, Beijing, China.
  • Liu X; The Key Laboratory of Plant-Soil Interactions, MOE, Department of Plant Nutrition, China Agricultural University, Beijing, China.
  • Zhao C; Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, ShanghaiChina.
  • Zhao J; Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, MOE Joint International Research Laboratory of Crop Molecular Breeding, China Agricultural University, BeijingChina.
  • Li M; The Key Laboratory of Plant-Soil Interactions, MOE, Department of Plant Nutrition, China Agricultural University, Beijing, China.
  • Ul Hassan M; The Key Laboratory of Plant-Soil Interactions, MOE, Department of Plant Nutrition, China Agricultural University, Beijing, China.
  • Yang B; State Key Laboratory of Plant physiology and Biochemistry and National Centre of Maize Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, BeijingChina.
  • Li D; Department of Crop Genomics and Bioinformatics, National Centre of Maize Genetic Improvement, China Agricultural University, BeijingChina.
  • Liu R; Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology, Fujian Agricultural and Forestry University, FuzhouChina.
  • Li X; The Key Laboratory of Plant-Soil Interactions, MOE, Department of Plant Nutrition, China Agricultural University, Beijing, China.
J Exp Bot ; 72(10): 3846-3863, 2021 05 04.
Article en En | MEDLINE | ID: mdl-33765129
Phosphorus and nitrogen nutrition have profound and complicated innate connections; however, underlying molecular mechanisms are mostly elusive. PHR1 is a master phosphate signaling component, and whether it directly functions in phosphorus-nitrogen crosstalk remains a particularly interesting question. In maize, nitrogen limitation caused tip kernel abortion and ear shortening. By contrast, moderately low phosphate in the field reduced kernels across the ear, maintained ear elongation and significantly lowered concentrations of total free amino acids and soluble proteins 2 weeks after silking. Transcriptome profiling revealed significant enrichment and overall down-regulation of transport genes in ears under low phosphate. Importantly, 313 out of 847 differentially expressed genes harbored PHR1 binding sequences (P1BS) including those controlling amino acid/polyamine transport and metabolism. Specifically, both ZmAAP2 and ZmLHT1 are plasma membrane-localized broad-spectrum amino acid transporters, and ZmPHR1.1 and ZmPHR1.2 were able to bind to P1BS-containing ZmAAP2 and ZmLHT1 and down-regulate their expression in planta. Taken together, the results suggest that prevalence of P1BS elements enables ZmPHR1s to regulate a large number of low phosphate responsive genes. Further, consistent with reduced accumulation of free amino acids, ZmPHR1s down-regulate ZmAAP2 and ZmLHT1 expression as direct linkers of phosphorus and nitrogen nutrition independent of NIGT1 in maize ear under low phosphate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Zea mays Tipo de estudio: Risk_factors_studies Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Zea mays Tipo de estudio: Risk_factors_studies Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido