Your browser doesn't support javascript.
loading
Facile modification of polycaprolactone nanofibers with egg white protein.
Renkler, Nergis Zeynep; Ergene, Emre; Gokyer, Seyda; Tuzlakoglu Ozturk, Merve; Yilgor Huri, Pinar; Tuzlakoglu, Kadriye.
Afiliación
  • Renkler NZ; Department of Polymer Engineering, Yalova University, 77200, Yalova, Turkey. nzrenkler@gmail.com.
  • Ergene E; Department of Biomedical Engineering, Ankara University, Ankara, Turkey.
  • Gokyer S; Department of Biomedical Engineering, Ankara University, Ankara, Turkey.
  • Tuzlakoglu Ozturk M; Department of Molecular Biology and Genetics, Gebze Technical University, Kocaeli, Turkey.
  • Yilgor Huri P; Department of Biomedical Engineering, Ankara University, Ankara, Turkey.
  • Tuzlakoglu K; Department of Polymer Engineering, Yalova University, 77200, Yalova, Turkey.
J Mater Sci Mater Med ; 32(4): 34, 2021 Mar 24.
Article en En | MEDLINE | ID: mdl-33763760
Synthetic polymers remain to be a major choice for scaffold fabrication due to their structural stability and mechanical strength. However, the lack of functional moieties limits their application for cell-based therapies which necessitate modification and functionalization. Blending synthetic polymers with natural components is a simple and effective way to achieve the desired biological properties for a scaffold. Herein, nanofibrous mats made of polycaprolactone (PCL) and egg white protein (EWP) blend were developed and further evaluated for use as a scaffold for tissue engineering applications. Homogeneous distribution of EWP was achieved throughout the nanofibrous mats, as shown by immunohistochemistry. ATR-FTIR analysis and contact angle measurements have further confirmed the presence of EWP on the surface of the samples. The swelling test showed that PCL/EWP nanofibers have higher water uptake than PCL nanofibrous mats. Also, EWP addition on the nanofibrous mats resulted in an increase in the tensile strength and Young's modulus of the mats, indicating that the presence of protein can greatly enhance the mechanical properties of the mats. A significantly higher, more uniform, and dispersed cell spreading was observed on days 7 and 14 than that on neat PCL mats, demonstrating the importance of providing the required cues for cell homing by the availability of EWP. Hence, EWP is shown to be a simple and low-cost source for the functionalization of PCL nanofibrous mats. EWP is, therefore, a facile candidate to enhance cellular interactions of synthetic polymers for a wide range of tissue engineering applications.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliésteres / Polímeros / Proteínas del Huevo / Ingeniería de Tejidos / Nanofibras Límite: Animals / Humans Idioma: En Revista: J Mater Sci Mater Med Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliésteres / Polímeros / Proteínas del Huevo / Ingeniería de Tejidos / Nanofibras Límite: Animals / Humans Idioma: En Revista: J Mater Sci Mater Med Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos