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Effects of storage and leukocyte reduction on the concentration and procoagulant activity of extracellular vesicles in canine packed red cells.
Avenick, Daniel; Kidd, Linda; Istvan, Stephanie; Dong, Fanglong; Richter, Keith; Edwards, Nicole; Hisada, Yohei; Posma, Jens J N; Massih, Cherein Abdel; Mackman, Nigel.
Afiliación
  • Avenick D; Veterinary Specialty Hospital, San Diego, California.
  • Kidd L; College of Veterinary Medicine, Western University of Health Sciences, Pomona, California.
  • Istvan S; Veterinary Specialty Hospital, San Diego, California.
  • Dong F; Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, California.
  • Richter K; Veterinary Specialty Hospital, San Diego, California.
  • Edwards N; Veterinary Specialty Hospital, San Diego, California.
  • Hisada Y; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
  • Posma JJN; Departments of Internal Medicine and Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.
  • Massih CA; College of Veterinary Medicine, Western University of Health Sciences, Pomona, California.
  • Mackman N; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
J Vet Emerg Crit Care (San Antonio) ; 31(2): 221-230, 2021 Mar.
Article en En | MEDLINE | ID: mdl-33751799
OBJECTIVES: To characterize the size and procoagulant activity of extracellular vesicles (EV) that accumulate in canine packed red blood cells (pRBCs) over time and the effect of leukocyte reduction on these characteristics. DESIGN: Prospective cohort study. SETTING: Private small animal specialty referral hospital and university research laboratories. ANIMALS: Ten healthy blood donor dogs. INTERVENTIONS: Five pRBCs units were obtained according to standard protocols, and 5 were leukocyte-reduced prior to processing. Platelet-free supernatant from the pRBC units was collected on days 0, 10, 20, 32, and 42. MEASUREMENTS AND MAIN RESULTS: Nanoparticle tracking analysis was performed to determine the size and concentration of EVs. Thrombin generation associated with phosphatidylserine-positive EVs was determined using a capture assay. Factor Xa generation associated with phosphatidylserine-positive EVs and tissue factor-positive EVs was measured in a subset of EVs isolated by centrifugation of the supernatant at 20,000 × g. R package nparLD and the Mann-Whitney U-test were used to determine the effect of duration of storage and the effect of leukocyte reduction, respectively. Small (mean < 125 nm) procoagulant EVs accumulated over time, with significant increases occurring on or after day 20 in both non-leukocyte reduced and leukocyte-reduced units. The procoagulant activity of the EVs was due to phosphatidylserine, not tissue factor. Increases in EV concentration and procoagulant activity occurred earlier in non-leukocyte reduced units. Extracellular vesicle accumulation and procoagulant activity were not decreased at any individual time point by leukocyte reduction. CONCLUSIONS: Further studies characterizing and determining the clinical relevance of small procoagulant EVs in pRBCs are warranted.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tromboplastina / Perros / Eritrocitos / Vesículas Extracelulares / Leucocitos Tipo de estudio: Etiology_studies / Guideline / Incidence_studies / Observational_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: J Vet Emerg Crit Care (San Antonio) Asunto de la revista: MEDICINA DE EMERGENCIA / MEDICINA VETERINARIA / TERAPIA INTENSIVA Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tromboplastina / Perros / Eritrocitos / Vesículas Extracelulares / Leucocitos Tipo de estudio: Etiology_studies / Guideline / Incidence_studies / Observational_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: J Vet Emerg Crit Care (San Antonio) Asunto de la revista: MEDICINA DE EMERGENCIA / MEDICINA VETERINARIA / TERAPIA INTENSIVA Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos