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Extracts from Dated Lake Sediment Cores in the Athabasca Oil Sands Region Alter Ethoxyresorufin-O-deethylase Activity and Gene Expression in Avian Hepatocytes.
Salat, Alexandre P J; Williams, Kim L; Chiu, Suzanne; Eickmeyer, David C; Kimpe, Linda E; Blais, Jules M; Crump, Doug.
Afiliación
  • Salat APJ; Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.
  • Williams KL; Ecotoxicology and Wildlife Health Division, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada.
  • Chiu S; Ecotoxicology and Wildlife Health Division, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada.
  • Eickmeyer DC; Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.
  • Kimpe LE; Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.
  • Blais JM; Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.
  • Crump D; Ecotoxicology and Wildlife Health Division, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada.
Environ Toxicol Chem ; 40(7): 1883-1893, 2021 07.
Article en En | MEDLINE | ID: mdl-33751657
Increases in oil sands mining operations in the Athabasca oil sands region have resulted in increased concentrations of polycyclic aromatic compounds (PACs) and heavy metals in aquatic systems located near surface mining operations. In the present study, sediment cores were collected from 3 lakes with varying proximity to surface mining operations to determine the differences in PAC concentrations. Sediment cores were separated into 2 sections-current mining (top; 2000-2017) and premining (bottom; pre-1945)-and extracts were prepared for in vitro screening using a well-established chicken embryonic hepatocyte (CEH) assay. Concentrations and composition of PACs varied between sites, with the highest ∑PACs in Saline Lake, 5 km from an active oil sands mine site. The proportion of alkylated PACs was greater than that of parent PACs in the top sediment sections compared with the bottom. Ethoxyresorufin-O-deethylase activity in CEH permitted the ranking of lake sites/core sections based on an aryl hydrocarbon receptor-mediated end point; mean median effect concentration values were lowest for the top cores from Saline Lake and another near-mining operations lake, referred to as WF1. A ToxChip polymerase chain reaction (PCR) array was used to evaluate gene expression changes across 43 target genes associated with numerous toxicological pathways following exposure to top and bottom sediment core extracts. The 2 study sites with the greatest ∑PAC concentrations (Saline Lake and WF1) had the highest gene expression alterations on the ToxChip PCR array (19 [top] and 17 [bottom]/43), compared with a reference site (13 [top] and 7 [bottom]/43). The avian in vitro bioassay was useful for identifying the toxicity of complex PAC extracts associated with variably contaminated sediment cores, supporting its potential use for hotspot identification and complex mixture screening. EnvironToxicol Chem 2021;40:1883-1893. © 2021 SETAC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes Químicos del Agua / Hepatocitos Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Environ Toxicol Chem Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes Químicos del Agua / Hepatocitos Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Environ Toxicol Chem Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos