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Aptamer Switches Regulated by Post-Transition/Transition Metal Ions.
Billet, Blandine; Chovelon, Benoit; Fiore, Emmanuelle; Oukacine, Farid; Petrillo, Mel-Alexandre; Faure, Patrice; Ravelet, Corinne; Peyrin, Eric.
Afiliación
  • Billet B; DPM UMR 5063, University Grenoble Alpes, CNRS, 38041, Grenoble, France.
  • Chovelon B; Biochemistry, Toxicology and Pharmacology Department, Grenoble site Nord CHU- Biology and Pathology Institute, 38041, Grenoble, France.
  • Fiore E; DPM UMR 5063, University Grenoble Alpes, CNRS, 38041, Grenoble, France.
  • Oukacine F; Biochemistry, Toxicology and Pharmacology Department, Grenoble site Nord CHU- Biology and Pathology Institute, 38041, Grenoble, France.
  • Petrillo MA; DPM UMR 5063, University Grenoble Alpes, CNRS, 38041, Grenoble, France.
  • Faure P; DPM UMR 5063, University Grenoble Alpes, CNRS, 38041, Grenoble, France.
  • Ravelet C; DPM UMR 5063, University Grenoble Alpes, CNRS, 38041, Grenoble, France.
  • Peyrin E; DPM UMR 5063, University Grenoble Alpes, CNRS, 38041, Grenoble, France.
Angew Chem Int Ed Engl ; 60(22): 12346-12350, 2021 05 25.
Article en En | MEDLINE | ID: mdl-33742515
We introduced an aptamer switch design that relies on the ability of post-transition/transition metal ions to trigger, through their coordination to nucleobases, substantial DNA destabilization. In the absence of molecular target, the addition of one such metal ion to usual aptamer working solutions promotes the formation of an alternative, inert DNA state. Upon exposure to the cognate compound, the equilibrium is shifted towards the competent DNA form. The switching process was preferentially activated by metal ions of intermediate base over phosphate complexation preference (i.e. Pb2+ , Cd2+ ) and operated with diversely structured DNA molecules. This very simple aptamer switch scheme was applied to the detection of small organics using the fluorescence anisotropy readout mode. We envision that the approach could be adapted to a variety of signalling methods that report on changes in the surface charge density of DNA receptors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Angew Chem Int Ed Engl Año: 2021 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Angew Chem Int Ed Engl Año: 2021 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Alemania