Multispectral compressive fluorescence lifetime imaging microscopy with a SPAD array detector.
Opt Lett
; 46(6): 1353-1356, 2021 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-33720185
Multispectral/hyperspectral fluorescence lifetime imaging microscopy (λFLIM) is a promising tool for studying functional and structural biological processes. The rich information content provided by a multidimensional dataset is often in contrast with the acquisition speed. In this work, we develop and experimentally demonstrate a wide-field λFLIM setup, based on a novel time-resolved 18×1 single-photon avalanche diode array detector working in a single-pixel camera scheme, which parallelizes the spectral detection, reducing measurement time. The proposed system, which implements a single-pixel camera with a compressive sensing scheme, represents an optimal microscopy framework towards the design of λFLIM setups.
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01-internacional
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MEDLINE
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En
Revista:
Opt Lett
Año:
2021
Tipo del documento:
Article
Pais de publicación:
Estados Unidos