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Single-Atom Ruthenium Biomimetic Enzyme for Simultaneous Electrochemical Detection of Dopamine and Uric Acid.
Xie, Xiaoli; Wang, Dong Ping; Guo, Chunxian; Liu, Yuhang; Rao, Qianghai; Lou, Fangming; Li, Qiannan; Dong, Yongqiang; Li, Qunfang; Yang, Hong Bin; Hu, Fang Xin.
Afiliación
  • Xie X; School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, P.R. China.
  • Wang DP; Institute of Materials Science & Devices, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
  • Guo C; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P.R. China.
  • Liu Y; Institute of Materials Science & Devices, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
  • Rao Q; Institute of Materials Science & Devices, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
  • Lou F; Institute of Materials Science & Devices, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
  • Li Q; School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, P.R. China.
  • Dong Y; School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, P.R. China.
  • Li Q; MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350000, P.R. China.
  • Yang HB; School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, P.R. China.
  • Hu FX; Institute of Materials Science & Devices, Suzhou University of Science and Technology, Suzhou 215009, P.R. China.
Anal Chem ; 93(11): 4916-4923, 2021 03 23.
Article en En | MEDLINE | ID: mdl-33719390
Single-atom catalysts have attracted numerous attention due to the high utilization of metallic atoms, abundant active sites, and highly catalytic activities. Herein, a single-atom ruthenium biomimetic enzyme (Ru-Ala-C3N4) is prepared by dispersing Ru atoms on a carbon nitride support for the simultaneous electrochemical detection of dopamine (DA) and uric acid (UA), which are coexisting important biological molecules involving in many physiological and pathological aspects. The morphology and elemental states of the single-atom Ru catalyst are studied by transmission electron microscopy, energy dispersive X-ray elemental mapping, high-angle annular dark field-scanning transmission electron microscopy, and high-resolution X-ray photoelectron spectroscopy. Results show that Ru atoms atomically disperse throughout the C3N4 support by Ru-N chemical bonds. The electrochemical characterizations indicate that the Ru-Ala-C3N4 biosensor can simultaneously detect the oxidation of DA and UA with a separation of peak potential of 180 mV with high sensitivity and excellent selectivity. The calibration curves for DA and UA range from 0.06 to 490 and 0.5 to 2135 µM with detection limits of 20 and 170 nM, respectively. Moreover, the biosensor was applied to detect DA and UA in real biological serum samples using the standard addition method with satisfactory results.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rutenio / Ácido Úrico Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chem Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rutenio / Ácido Úrico Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chem Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos