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Simultaneous Hydrolysis and Extraction Increased Erucin Yield from Broccoli Seeds.
Lv, Chengzhi; Zhang, Yao; Zou, Ligen; Sun, Juan; Song, Xinjie; Mao, Jianwei; Wu, Yuanfeng.
Afiliación
  • Lv C; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
  • Zhang Y; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
  • Zou L; Hangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
  • Sun J; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
  • Song X; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
  • Mao J; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
  • Wu Y; Zhejiang Provincial Key Laboratory for Chem and Bio Processing Technology of Farm Produces, Hangzhou, Zhejiang 310023, China.
ACS Omega ; 6(9): 6385-6392, 2021 Mar 09.
Article en En | MEDLINE | ID: mdl-33718729
Isothiocyanates (ITCs) are well-known chemopreventive agents that have received significant interest across the nutrition and pharmaceutical industries owing to their anticancer properties, thus it is essential to increase the conversion of glucosinolate to ITCs by myrosinase to maximize their health benefits. In this paper, using broccoli seed meals as a raw material, we comparatively analyzed the outcomes of two extraction methods: (i) hydrolysis followed by extraction (HFE) and (ii) simultaneous hydrolysis and extraction (SHE) in terms of the ITC yield. The results revealed that the SHE method showed a relatively greater erucin production from broccoli seeds and greater antitumor and antioxidant activities. A similar phenomenon was found for the hydrolysates of crude myrosinase and crude glucosinolate separated from broccoli seeds. However, when the crude glucosinolates were hydrolyzed by purified broccoli myrosinase, or when pure glucoraphanin was hydrolyzed by crude myrosinase, no significant effects were noted on the types and yields of ITCs between the SHE and HFE methods.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos