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Co-invalidation of Prnp and Sprn in FVB/N mice affects reproductive performances and highlight complex biological relationship between PrP and Shadoo.
Castille, Johan; Passet, Bruno; Makhzami, Samira; Vilotte, Marthe; Moazami-Goudarzi, Katayoun; Truchet, Sandrine; Daniel-Carlier, Nathalie; Gaillard, Anne-Laure; Andréoletti, Olivier; Vaiman, Daniel; Beauvallet, Christian; Vaiman, Anne; Floriot, Sandrine; Calvel, Pierre; Mouillet-Richard, Sophie; Duchesne, Amandine; Béringue, Vincent; Vilotte, Jean-Luc.
Afiliación
  • Castille J; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: johan.castille@inrae.fr.
  • Passet B; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: bruno.passet@inrae.fr.
  • Makhzami S; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: samira.makhzami@kuleuven.vib.be.
  • Vilotte M; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: marthe.vilotte@inrae.fr.
  • Moazami-Goudarzi K; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: Katayoun.goudarzi@inrae.fr.
  • Truchet S; University Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France. Electronic address: sandrine.truchet@inrae.fr.
  • Daniel-Carlier N; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: nathalie.daniel-carlier@inrae.fr.
  • Gaillard AL; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: anne-laure.gaillard@mnhn.fr.
  • Andréoletti O; ISC3T, UMR1225, INRAE, ENVT, 31076, Toulouse Cedex, France. Electronic address: olivier.andreoletti@envt.fr.
  • Vaiman D; Institut Cochin, U1016, INSERM, UMR8504 CNRS, Université de Paris, Paris, France. Electronic address: daniel.vaiman@inserm.fr.
  • Beauvallet C; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: christian.beauvallet@inrae.fr.
  • Vaiman A; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: anne.vaiman@inrae.fr.
  • Floriot S; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: sandrine.floriot@inrae.fr.
  • Calvel P; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: pierre.calvel@agroparistech.fr.
  • Mouillet-Richard S; Centre de Recherche des Cordeliers, INSERM, Sorbonne University, 75006, Paris, France. Electronic address: sophie.mouillet-richard@parisdescartes.fr.
  • Duchesne A; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: amandine.duchesne@inrae.fr.
  • Béringue V; University Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France. Electronic address: vincent.beringue@inrae.fr.
  • Vilotte JL; University Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. Electronic address: jean-luc.vilotte@inrae.fr.
Biochem Biophys Res Commun ; 551: 1-6, 2021 04 30.
Article en En | MEDLINE | ID: mdl-33713980
Shadoo and PrP belongs to the same protein family, whose biological function remains poorly understood. Previous experiments reported potential functional redundancies or antagonisms between these two proteins, depending on the tissue analysed. While knockdown experiments suggested the requirement of Shadoo in the absence of PrP during early mouse embryogenesis, knockout ones, on the contrary, highlighted little impact, if any, of the double-knockout of these two loci. In the present study, we reinvestigated the phenotype associated with the concomitant knockout of these two genes using newly produced FVB/N Sprn knockout mice. In this genetic background, the combined two genes' knockout induces intra-uterine growth retardations, likely resulting from placental failures highlighted by transcriptomic analyses that revealed potential redundant or antagonist roles of these two proteins in different developmental-related pathways. It also induced an increased perinatal-lethality and ascertained the role of these two loci in the lactation process.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reproducción / Proteínas Priónicas / Proteínas del Tejido Nervioso Límite: Animals / Pregnancy Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reproducción / Proteínas Priónicas / Proteínas del Tejido Nervioso Límite: Animals / Pregnancy Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos