Your browser doesn't support javascript.
loading
Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma.
Yang, Yang; Wang, Yaojie; Wei, Sisi; Zhou, Chaoxi; Yu, Jiarui; Wang, Guiying; Wang, Wenxi; Zhao, Lianmei.
Afiliación
  • Yang Y; Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China.
  • Wang Y; Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China.
  • Wei S; Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China.
  • Zhou C; Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China.
  • Yu J; Department of Radiation Oncology, North China University of Science and Technology Affiliated People's Hospital, Tangshan, 063000, Hebei, China.
  • Wang G; Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China.
  • Wang W; Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China. wenxi0316@163.com.
  • Zhao L; Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China. zhaolianmei@hbydsy.com.
J Transl Med ; 19(1): 104, 2021 03 12.
Article en En | MEDLINE | ID: mdl-33712033
BACKGROUND: Extracellular vesicles (EVs), known as cell-derived membranous structures harboring a variety of biomolecules, have been widely used in liquid biopsy. Due to the complex biological composition of plasma, plasma RNA omics analysis (RNomics) is easily affected, thus it is necessary to select an optimal strategy from exiting methods according to the performance for intended application. METHODS: In this study, four different strategies for EVs isolation were performed and compared (i.e. ultracentrifugation (UC), size exclusion chromatography (SEC), and two most frequently-used commercially available isolation kit (ExoQuick and exoEasy). We compared the yield, purity, PCR quantification of RNAs, miRNA-seq analyses and mRNA-seq analyses of RNAs from EVs isolated using four methods. RESULTS: The results showed that the lowest miRNA binding protein AGO2 (Argonaute-2) and the highest EVs-specific miRNA and lncRNA were observed in EVs obtained through SEC, meanwhile the content of the non-specific miRNA was the lowest. Further RNA-Seq data revealed that RNAs obtained via SEC presented more useful reads for both miRNA and mRNA. Furthermore, the mRNA delivered via SEC tended to have a concentration comparable to the ideal FPKM (Fragments Per Kilobase Million) value. CONCLUSIONS: SEC shall be used as an optimal strategy for the isolation of EVs in plasma RNomics analysis.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / MicroARNs / Vesículas Extracelulares Límite: Humans Idioma: En Revista: J Transl Med Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / MicroARNs / Vesículas Extracelulares Límite: Humans Idioma: En Revista: J Transl Med Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido