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Hierarchical multi-layered scaffolds based on electrofluidodynamic processes for tissue engineering.
Piegat, Agnieszka; Niemczyk, Agata; Boccaccini, Aldo R; El Fray, Miroslawa; Liverani, Liliana.
Afiliación
  • Piegat A; Department of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, 45 Piastow Ave, 70-311 Szczecin, Poland.
  • Niemczyk A; Department of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, 45 Piastow Ave, 70-311 Szczecin, Poland.
  • Boccaccini AR; Department of Materials Technology, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology, 19 Piastow Ave, 70-310 Szczecin, Poland.
  • El Fray M; Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, Germany.
  • Liverani L; Department of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, 45 Piastow Ave, 70-311 Szczecin, Poland.
Biomed Mater ; 16(4)2021 03 22.
Article en En | MEDLINE | ID: mdl-33691298
The aim of this study was to obtain hierarchical scaffolds combining 3D printing and two electrofluidodynamic methods. The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA). Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process. The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate. By using the optimized electrospraying conditions, uniformly distributed spheres have been obtained, decorating struts and nanofibers. Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy. Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding. Moreover, the addition of the electrosprayed nanoparticles does not affect the cell seeding efficiency. The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Nanofibras Límite: Animals Idioma: En Revista: Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Nanofibras Límite: Animals Idioma: En Revista: Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido