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Efficient preparation of dual-emission ratiometric fluorescence sensor system based on aptamer-composite and detection of bis(2-ethylhexyl) phthalate in pork.
Wang, Yueying; Li, Wenting; Hu, Xuetao; Zhang, Xinai; Huang, Xiaowei; Li, Zhihua; Li, Miaoyun; Zou, Xiaobo; Shi, Jiyong.
Afiliación
  • Wang Y; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Li W; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Hu X; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Zhang X; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Huang X; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Li Z; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Li M; College of Food Science and Technology, Henan Meat Processing and Safety International United Lab, Henan Agricultural University, Zhengzhou 450000, China. Electronic address: Limy7476@126.com.
  • Zou X; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
  • Shi J; Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address: shi_jiyong@ujs.edu.cn.
Food Chem ; 352: 129352, 2021 Aug 01.
Article en En | MEDLINE | ID: mdl-33691206
A ratiometric fluorescence sensor system is proposed for detecting bis(2-ethylhexyl) phthalate (DEHP) in pork, which is based on aptamer recognition with molybdenum disulfide quantum dots and cadmium telluride quantum dots (MoS2 QDs/CdTe-Apta). Two signals exist in the system, among which the response signal is transmitted by CdTe-Apta. The amide condensation between aptamers and CdTe QDs shortens the distance between CdTe QDs and DEHP, thus quenching the fluorescence of CdTe QDs, possibly through a photoinduced electron transfer mechanism. The MoS2 QDs deliver the self-calibration signal, and the fluorescence of MoS2 QDs remains almost constant when co-existing with DEHP. Linearity (R2 = 0.9536) was established for the DEHP concentration range 0.005-3.0 mg·L-1, with a limit of detection of 0.21 µg·L-1. The system was successfully applied in the determination of DEHP in pork. The system has potential for the quantitative determination of DEHP in practical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Dietilhexil Ftalato / Aptámeros de Nucleótidos / Carne de Cerdo Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Food Chem Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Dietilhexil Ftalato / Aptámeros de Nucleótidos / Carne de Cerdo Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Food Chem Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido