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Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system.
Shinohara, Marie; Arakawa, Hiroshi; Oda, Yuuichi; Shiraki, Nobuaki; Sugiura, Shinji; Nishiuchi, Takumi; Satoh, Taku; Iino, Keita; Leo, Sylvia; Kato, Yusuke; Araya, Karin; Kawanishi, Takumi; Nakatsuji, Tomoki; Mitsuta, Manami; Inamura, Kosuke; Goto, Tomomi; Shinha, Kenta; Nihei, Wataru; Komori, Kikuo; Nishikawa, Masaki; Kume, Shoen; Kato, Yukio; Kanamori, Toshiyuki; Sakai, Yasuyuki; Kimura, Hiroshi.
Afiliación
  • Shinohara M; Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
  • Arakawa H; Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Oda Y; Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
  • Shiraki N; Department of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
  • Sugiura S; School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
  • Nishiuchi T; Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
  • Satoh T; Advanced Science Research Centre, Kanazawa University, Kanazawa, Japan.
  • Iino K; Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
  • Leo S; School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
  • Kato Y; School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
  • Araya K; School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
  • Kawanishi T; Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Nakatsuji T; Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Mitsuta M; Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Inamura K; Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
  • Goto T; Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
  • Shinha K; Department of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
  • Nihei W; Department of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
  • Komori K; Department of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
  • Nishikawa M; Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
  • Kume S; Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
  • Kato Y; School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
  • Kanamori T; Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Sakai Y; Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
  • Kimura H; Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Sci Rep ; 11(1): 5437, 2021 03 08.
Article en En | MEDLINE | ID: mdl-33686099
Examining intestine-liver interactions is important for achieving the desired physiological drug absorption and metabolism response in in vitro drug tests. Multi-organ microphysiological systems (MPSs) constitute promising tools for evaluating inter-organ interactions in vitro. For coculture on MPSs, normal cells are challenging to use because they require complex maintenance and careful handling. Herein, we demonstrated the potential of coculturing normal cells on MPSs in the evaluation of intestine-liver interactions. To this end, we cocultured human-induced pluripotent stem cell-derived intestinal cells and fresh human hepatocytes which were isolated from PXB mice with medium circulation in a pneumatic-pressure-driven MPS with pipette-friendly liquid-handling options. The cytochrome activity, albumin production, and liver-specific gene expressions in human hepatocytes freshly isolated from a PXB mouse were significantly upregulated via coculture with hiPS-intestinal cells. Our normal cell coculture shows the effects of the interactions between the intestine and liver that may occur in vivo. This study is the first to demonstrate the coculturing of hiPS-intestinal cells and fresh human hepatocytes on an MPS for examining pure inter-organ interactions. Normal-cell coculture using the multi-organ MPS could be pursued to explore unknown physiological mechanisms of inter-organ interactions in vitro and investigate the physiological response of new drugs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepatocitos / Técnicas Analíticas Microfluídicas / Células Madre Pluripotentes Inducidas / Dispositivos Laboratorio en un Chip Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepatocitos / Técnicas Analíticas Microfluídicas / Células Madre Pluripotentes Inducidas / Dispositivos Laboratorio en un Chip Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido