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Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater.
Dumke, Roger; de la Cruz Barron, Magali; Oertel, Reinhard; Helm, Björn; Kallies, Rene; Berendonk, Thomas U; Dalpke, Alexander.
Afiliación
  • Dumke R; Institute of Medical Microbiology and Virology, Technische Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany.
  • de la Cruz Barron M; Institute of Hydrobiology, Technische Universität Dresden, Helmholtzstrasse 10, 01069 Dresden, Germany.
  • Oertel R; Department of River Ecology, Helmholtz Centre for Environmental Research-UFZ, Brückstrasse 3a, 39114 Magdeburg, Germany.
  • Helm B; Institute of Clinical Pharmacology, Technische Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany.
  • Kallies R; Institute of Urban and Industrial Water Management, Technische Universität Dresden, Helmholtzstrasse 10, 01069 Dresden, Germany.
  • Berendonk TU; Department of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstrasse 15, 04318 Leipzig, Germany.
  • Dalpke A; Institute of Hydrobiology, Technische Universität Dresden, Helmholtzstrasse 10, 01069 Dresden, Germany.
Pathogens ; 10(2)2021 Feb 12.
Article en En | MEDLINE | ID: mdl-33673032
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pathogens Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pathogens Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza