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Platelet lysates-based hydrogels incorporating bioactive mesoporous silica nanoparticles for stem cell osteogenic differentiation.
Tavares, M T; Santos, S C; Custódio, C A; Farinha, J P S; Baleizão, C; Mano, J F.
Afiliación
  • Tavares MT; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
  • Santos SC; CQE - Centro de Química Estrutural, IN-Institute of Nanosciences and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  • Custódio CA; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
  • Farinha JPS; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
  • Baleizão C; CQE - Centro de Química Estrutural, IN-Institute of Nanosciences and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  • Mano JF; CQE - Centro de Química Estrutural, IN-Institute of Nanosciences and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
Mater Today Bio ; 9: 100096, 2021 Jan.
Article en En | MEDLINE | ID: mdl-33665604
Scaffolds for bone tissue regeneration should provide the right cues for stem cell adhesion and proliferation, but also lead to their osteogenic differentiation. Hydrogels of modified platelet lysates (PLMA) show the proper mechanical stability for cell encapsulation and contain essential bioactive molecules required for cell maintenance. We prepared a novel PLMA-based nanocomposite for bone repair and regeneration capable of releasing biofactors to induce osteogenic differentiation. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were encapsulated in PLMA hydrogels containing bioactive mesoporous silica nanoparticles previously loaded with dexamethasone and functionalized with calcium and phosphate ions. After 21 d of culture, hBM-MSCs remained viable, presented a stretched morphology, and showed signs of osteogenic differentiation, namely the presence of significant amounts of alkaline phosphatase, bone morphogenic protein-2 and osteopontin, hydroxyapatite, and calcium nodules. Developed for the first time, PLMA/MSNCaPDex nanocomposites were able to guide the differentiation of hBM-MSCs without any other osteogenic supplementation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mater Today Bio Año: 2021 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mater Today Bio Año: 2021 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido