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Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications.
Bhatwa, Arshpreet; Wang, Weijun; Hassan, Yousef I; Abraham, Nadine; Li, Xiu-Zhen; Zhou, Ting.
Afiliación
  • Bhatwa A; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
  • Wang W; Department of Biology, University of Waterloo, Waterloo, ON, Canada.
  • Hassan YI; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
  • Abraham N; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
  • Li XZ; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
  • Zhou T; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.
Front Bioeng Biotechnol ; 9: 630551, 2021.
Article en En | MEDLINE | ID: mdl-33644021
Recombinant proteins are becoming increasingly important for industrial applications, where Escherichia coli is the most widely used bacterial host for their production. However, the formation of inclusion bodies is a frequently encountered challenge for producing soluble and functional recombinant proteins. To overcome this hurdle, different strategies have been developed through adjusting growth conditions, engineering host strains of E. coli, altering expression vectors, and modifying the proteins of interest. These approaches will be comprehensively highlighted with some of the new developments in this review. Additionally, the unique features of protein inclusion bodies, the mechanism and influencing factors of their formation, and their potential advantages will also be discussed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Front Bioeng Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Front Bioeng Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza