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Size controlled fabrication of enzyme encapsulated amorphous calcium phosphate nanoparticle and its intracellular biosensing application.
Luo, Ying; Zhao, Jiaqian; Zhang, Xinran; Wang, Chengcheng; Wang, Tongyu; Jiang, Min; Zhu, Qin; Xie, Tian; Chen, Dajing.
Afiliación
  • Luo Y; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, China; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Zhao J; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Zhang X; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Wang C; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Wang T; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Jiang M; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Zhu Q; College of Pharmacy, School of Medicine, Hangzhou Normal University, China.
  • Xie T; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, China; College of Pharmacy, School of Medicine, Hangzhou Normal University, China. Electronic address: xbs@hznu.edu.cn.
  • Chen D; College of Pharmacy, School of Medicine, Hangzhou Normal University, China. Electronic address: djchen@hznu.edu.cn.
Colloids Surf B Biointerfaces ; 201: 111638, 2021 May.
Article en En | MEDLINE | ID: mdl-33639505
Inorganic-enzyme composites have been widely used for applications in catalysis and analytical science. Amorphous calcium phosphate, as a biocompatible material, can form open hydrated structure to encapsulate and protect enzymes. So far, there have been few progress on size-adjustable amorphous calcium phosphate nanoparticles since the diameter controllability is limited by its natural aggregation characteristics. By co-precipitation and nano-channel extrusion, we developed enzyme-loaded amorphous calcium phosphate nanoparticles with adjustable diameters. These enzyme-loaded particles showed high thermal and chemical stability as well as biocompatibility. The nano-sized enzyme-loaded particles can further expand their application fields and be used as intracellular enzyme probes. Delivering glucose oxidase enzyme by amorphous calcium phosphate nanoparticles enables fluorescent monitoring of glucose levels in living cells, which can be used to study the metabolism rates of cancer cells and normal cells. The nano-channel extrusion method can also be used as a template to encapsulate different kinds of enzymes to expand catalysis and biosensing applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos de Calcio / Nanopartículas Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos de Calcio / Nanopartículas Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos