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Assessment of corn starch as substitute for agarose in DNA gel electrophoresis.
Djankpa, Francis Tanam; Wiafe, Gideon Akuamoah; Boateng, Bernard Ntim; Tsegah, Korantema Mawuena; Essien-Baidoo, Samuel; Ulanja, Mark Bilinyi; Affram, Kwame Ofori; Ussif, Abdala Mumuni; Agyeman, Desmond Owusu; Asante, Gabriel.
Afiliación
  • Djankpa FT; Department of Physiology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana. fdjankpa@ucc.edu.gh.
  • Wiafe GA; Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
  • Boateng BN; Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
  • Tsegah KM; Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
  • Essien-Baidoo S; Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
  • Ulanja MB; Department of Internal Medicine, University of Nevada, Reno School of Medicine, 1155 Mill St, Reno, NV, 89502, USA.
  • Affram KO; Piedmont Athens Regional Medical Center, Graduate Medical Education, Athens, GA, USA.
  • Ussif AM; Department of Forensic Sciences, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana.
  • Agyeman DO; Department of Physiology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
  • Asante G; Department of Physiology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
BMC Res Notes ; 14(1): 73, 2021 Feb 25.
Article en En | MEDLINE | ID: mdl-33632279
OBJECTIVE: The use of agarose in nucleic acid electrophoresis is the gold standard. However, agarose is very expensive and not readily available in resource limited developing countries like Ghana. Hence, finding a more affordable and readily available alternative to agarose will be a major boost to molecular research in developing countries. This study was aimed at investigating the use of corn starch as a potential substitute for agarose in DNA gel electrophoresis. RESULTS: Genomic deoxyribonucleic acid (DNA) extracted from Plasmodium falciparum and primers were obtained from the West African Centre for Cell Biology of Infectious Pathogens and amplified using polymerase chain reaction. The amplicon was run on agarose gel to ascertain the molecular weight (as a positive control). When visualized under both blue light and ultraviolet light, the DNA and ladder showed clear and clean bands with the expected molecular weight. Corn starch was then modified with sodium borate buffer, casted into a gel and used to run the same DNA sample. Our findings indicated that similar to agarose, the DNA sample and ladder migrated successfully through the modified starch gel but no bands were visible when visualized under blue and ultra-violet light.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Almidón / Zea mays País/Región como asunto: Africa Idioma: En Revista: BMC Res Notes Año: 2021 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Almidón / Zea mays País/Región como asunto: Africa Idioma: En Revista: BMC Res Notes Año: 2021 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Reino Unido