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Discovery of a Macropinocytosis-Inducing Peptide Potentiated by Medium-Mediated Intramolecular Disulfide Formation.
Arafiles, Jan Vincent V; Hirose, Hisaaki; Hirai, Yusuke; Kuriyama, Masashi; Sakyiamah, Maxwell Mamfe; Nomura, Wataru; Sonomura, Kazuhiro; Imanishi, Miki; Otaka, Akira; Tamamura, Hirokazu; Futaki, Shiroh.
Afiliación
  • Arafiles JVV; Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
  • Hirose H; Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
  • Hirai Y; Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
  • Kuriyama M; Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
  • Sakyiamah MM; Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo, 101-0062, Japan.
  • Nomura W; Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo, 101-0062, Japan.
  • Sonomura K; Current address: Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
  • Imanishi M; Center for Genomic Medicine, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan.
  • Otaka A; Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation, Kyoto, 604-8445, Japan.
  • Tamamura H; Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
  • Futaki S; Institute of Biomedical Sciences, Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima, 770-8505, Japan.
Angew Chem Int Ed Engl ; 60(21): 11928-11936, 2021 05 17.
Article en En | MEDLINE | ID: mdl-33629482
Macropinocytosis is a ubiquitous cellular uptake mechanism of peptide-based intracellular delivery. This entry pathway shows promise as a route for the intracellular uptake of biomacromolecules and nanoparticles. In this work, we obtained the 8-residue analogue P4A bearing higher macropinocytosis induction ability. P4A contains vital cysteine residues in its sequence, which immediately reacts with cystine in culture medium to convert into its oxidized forms, including the intramolecularly oxidized form (oxP4A) as the dominant and active species. The conjugate of oxP4A and the membrane lytic peptide LK15 delivered bioactive proteins into cells; notably, this peptide delivered functional proteins fused with a negatively charged protein tag at a significantly reduced amount (up to nanomolar range) without compromising the delivery efficiency and the cellular activities of delivered proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Pinocitosis / Transporte de Proteínas Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Pinocitosis / Transporte de Proteínas Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania