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The effect of Q-switched 1064-nm Nd: YAG laser on skin barrier and collagen synthesis via miR-663a to regulate TGFß1/smad3/p38MAPK pathway.
Yang, Zhi; Duan, Xiaoxia; Wang, Xue; Xu, Qi; Guo, Birun; Xiang, Shunli; Jia, Xiaorong; He, Li.
Afiliación
  • Yang Z; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Duan X; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Wang X; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Xu Q; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Guo B; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Xiang S; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Jia X; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • He L; Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
Photodermatol Photoimmunol Photomed ; 37(5): 412-421, 2021 Sep.
Article en En | MEDLINE | ID: mdl-33621359
BACKGROUND: Our previous research found that Q-switched 1064-nm Nd: YAG laser (1064-QSNYL) induces skin collagen synthesis by activating TGFß1/Smad3/p38MAPKs pathway. Moreover, a lot of studies shown that MicroRNAs (miRNAs) contribute to regulate collagen synthesis and skin barrier. Therefore, we intend to explore the mechanism of 1064-QSNYL on collagen synthesis and skin barrier through miRNAs. METHODS: We predicted the upstream miRNAs of TGFß1 by bioinformatics databases, and verified them through dual-luciferase reporter genes and Western blotting. The expression of collagen, skin barrier-related protein K10 and filaggrin, TIMP-1, and MMP-2 were detected by RT-qPCR and Western blotting, respectively. Moreover, we detected moisture content, elasticity value, TEWL value, SOD vitality, and hydroxyproline content to evaluate skin barrier of mice. H&E staining to observe the change of dermis thickness and inflammation and infiltration of mice skin. RESULTS: The results shown that TGFß1 was target gene of miR-663a. Moreover, we found that 1064-QSNYL activated TGFß1/smad3/p38MAPK pathway by down-regulating the expression of miR-663a in HaCaT, HDF cells, and mice, thereby promoting expression of Collagen I, Collagen IV, TIMP-1, K10, and filaggrin and inhibiting MMP-2. Furthermore, 1064-QSNYL contributed to moisture content, elasticity, SOD vitality, and hydroxyproline content via miR-663a to activate TGFß1/smad3/p38MAPK pathway. CONCLUSIONS: In summary, this study found for the first time that 1064-QSNYL contributed to collagen synthesis and skin repair via miR-663a to regulate TGFß1/smad3/p38MAPK pathway, thereby achieving skin rejuvenation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Láseres de Estado Sólido Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Photodermatol Photoimmunol Photomed Asunto de la revista: ALERGIA E IMUNOLOGIA / DERMATOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Láseres de Estado Sólido Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Photodermatol Photoimmunol Photomed Asunto de la revista: ALERGIA E IMUNOLOGIA / DERMATOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido