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Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC.
Shamshad, Hina; Sayqal, Ali; Zeb, Jahan; Mirza, Agha Zeeshan.
Afiliación
  • Shamshad H; Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan.
  • Sayqal A; Chemistry Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, 21955 Saudi Arabia.
  • Zeb J; Department of Environmental and Health Research, The Custodian of the Two Holy Mosques, Institute of Hajj and Umrah Research, Umm Al-Qura University, Makkah, 21955 Saudi Arabia.
  • Mirza AZ; Chemistry Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, 21955 Saudi Arabia.
J Chromatogr Sci ; 59(10): 923-927, 2021 Oct 29.
Article en En | MEDLINE | ID: mdl-33621328
A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1-100 µg mL-1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pirimetamina / Cetirizina Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: J Chromatogr Sci Año: 2021 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pirimetamina / Cetirizina Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: J Chromatogr Sci Año: 2021 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Estados Unidos