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Evaluation of methods for detection of ß-lactamase production in MSSA.
Skov, Robert; Lonsway, David R; Larsen, Jesper; Larsen, Anders Rhod; Samulioniené, Jurgita; Limbago, Brandi M.
Afiliación
  • Skov R; Division of Infectious Disease Preparedness, Statens Serum Institut, Copenhagen, Denmark.
  • Lonsway DR; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Larsen J; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark.
  • Larsen AR; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark.
  • Samulioniené J; Department of Clinical Microbiology, Aalborg University Hospital, Alborg, Denmark.
  • Limbago BM; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA, USA.
J Antimicrob Chemother ; 76(6): 1487-1494, 2021 05 12.
Article en En | MEDLINE | ID: mdl-33615356
OBJECTIVES: Correct determination of penicillin susceptibility is pivotal for using penicillin in the treatment of Staphylococcus aureus infections. This study examines the performance of MIC determination, disc diffusion and a range of confirmatory tests for detection of penicillin susceptibility in S. aureus. METHODS: A total of 286 consecutive penicillin-susceptible S. aureus blood culture isolates as well as a challenge set of 62 MSSA isolates were investigated for the presence of the blaZ gene by PCR and subjected to penicillin-susceptibility testing using broth microdilution MIC determination, disc diffusion including reading of the zone edge, two nitrocefin tests and the cloverleaf test. RESULTS: Using PCR-based detection of blaZ as the gold standard, both broth microdilution MIC testing and disc diffusion testing resulted in a relatively low accuracy (82%-93%) with a sensitivity ranging from 49%-93%. Among the confirmatory tests, the cloverleaf test performed with 100% accuracy, while zone edge interpretation and nitrocefin-based tests increased the sensitivity of ß-lactamase detection to 96%-98% and 82%-96% when using MIC determination or disc diffusion as primary test, respectively. CONCLUSIONS: This investigation showed that reliable and accurate detection of ß-lactamase production in S. aureus can be obtained by MIC determination or penicillin disc diffusion followed by interpretation of the zone edge as a confirmatory test for apparently penicillin-susceptible isolates. The more cumbersome cloverleaf test can also be used. Nitrocefin-based tests should not be used as the only test for confirmation of a presumptive ß-lactamase-negative isolate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Beta-Lactamasas Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Antimicrob Chemother Año: 2021 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Beta-Lactamasas Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Antimicrob Chemother Año: 2021 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido