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Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2.
Monchatre-Leroy, Elodie; Lesellier, Sandrine; Wasniewski, Marine; Picard-Meyer, Evelyne; Richomme, Céline; Boué, Franck; Lacôte, Sandra; Murri, Séverine; Pulido, Coralie; Vulin, Johann; Salguero, Francisco J; Gouilh, Meriadeg Ar; Servat, Alexandre; Marianneau, Philippe.
Afiliación
  • Monchatre-Leroy E; Nancy laboratory for rabies and wildlife, ANSES, Malzéville, France.
  • Lesellier S; Nancy laboratory for rabies and wildlife, ANSES, Atton experimental facility, Atton France, France.
  • Wasniewski M; Nancy laboratory for rabies and wildlife, ANSES, Lyssavirus Unit, Malzéville, France.
  • Picard-Meyer E; Nancy laboratory for rabies and wildlife, ANSES, Lyssavirus Unit, Malzéville, France.
  • Richomme C; Nancy laboratory for rabies and wildlife, ANSES, SEEpiAS Unit, Malzéville, France.
  • Boué F; Nancy laboratory for rabies and wildlife, ANSES, SEEpiAS Unit, Malzéville, France.
  • Lacôte S; Lyon laboratory, ANSES, Virology Unit, Lyon, France.
  • Murri S; Lyon laboratory, ANSES, Virology Unit, Lyon, France.
  • Pulido C; Lyon laboratory, ANSES, Platform of animal experiment, Lyon, France.
  • Vulin J; Lyon laboratory, ANSES, Virology Unit, Lyon, France.
  • Salguero FJ; Public Health England, Porton Down, UK.
  • Gouilh MA; Service de Virologie, CHU de Caen, Caen, France.
  • Servat A; Groupe de Recherche sur l'Adaptation Microbienne - GRAM2, Université de Caen Normandie, Caen, France.
  • Marianneau P; Nancy laboratory for rabies and wildlife, ANSES, Lyssavirus Unit, Malzéville, France.
J Gen Virol ; 102(3)2021 03.
Article en En | MEDLINE | ID: mdl-33612147
Understanding the pathogenesis of the SARS-CoV-2 infection is key to developing preventive and therapeutic strategies against COVID-19, in the case of severe illness but also when the disease is mild. The use of appropriate experimental animal models remains central in the in vivo exploration of the physiopathology of infection and antiviral strategies. This study describes SARS-CoV-2 intranasal infection in ferrets and hamsters with low doses of low-passage SARS-CoV-2 clinical French isolate UCN19, describing infection levels, excretion, immune responses and pathological patterns in both animal species. Individual infection with 103 p.f.u. SARS-CoV-2 induced a more severe disease in hamsters than in ferrets. Viral RNA was detected in the lungs of hamsters but not of ferrets and in the brain (olfactory bulb and/or medulla oblongata) of both species. Overall, the clinical disease remained mild, with serological responses detected from 7 days and 10 days post-inoculation in hamsters and ferrets respectively. The virus became undetectable and pathology resolved within 14 days. The kinetics and levels of infection can be used in ferrets and hamsters as experimental models for understanding the pathogenicity of SARS-CoV-2, and testing the protective effect of drugs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cricetinae / Modelos Animales de Enfermedad / Hurones / COVID-19 / Anticuerpos Antivirales Límite: Animals Idioma: En Revista: J Gen Virol Año: 2021 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cricetinae / Modelos Animales de Enfermedad / Hurones / COVID-19 / Anticuerpos Antivirales Límite: Animals Idioma: En Revista: J Gen Virol Año: 2021 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido