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Cryopreservation and Transplantation of Spermatogonial Stem Cells.
Marinovic, Zoran; Lujic, Jelena; Li, Qian; Iwasaki, Yoshiko; Urbányi, Béla; Yoshizaki, Goro; Horváth, Ákos.
Afiliación
  • Marinovic Z; Department of Aquaculture, Szent István University, Gödöllo, Páter Károly u. 1., Hungary.
  • Lujic J; Department of Biomedical Sciences, Center for Reproductive Genomics, Cornell University, Ithaca, NY, USA.
  • Li Q; National Engineering Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, Shanghai, People's Republic of China.
  • Iwasaki Y; Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
  • Urbányi B; Department of Aquaculture, Szent István University, Gödöllo, Páter Károly u. 1., Hungary.
  • Yoshizaki G; Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
  • Horváth Á; Department of Aquaculture, Szent István University, Gödöllo, Páter Károly u. 1., Hungary. Horvath.Akos@szie.hu.
Methods Mol Biol ; 2218: 37-47, 2021.
Article en En | MEDLINE | ID: mdl-33606221
Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatogonias / Espermatozoides / Trasplante / Criopreservación / Células Madre Germinales Adultas Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatogonias / Espermatozoides / Trasplante / Criopreservación / Células Madre Germinales Adultas Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos