Biomimetic 3D-Bone Tissue Model.

Parmaksiz, Mahmut; Elçin, Ayse Eser; Elçin, Yasar Murat

Parmaksiz, Mahmut; Elçin, Ayse Eser; Elçin, Yasar Murat.

Afiliación

Parmaksiz M; Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey.
Elçin AE; Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey.
Elçin YM; Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey. elcinmurat@gmail.com.

Methods Mol Biol ; 2273: 239-250, 2021.

Article en En | MEDLINE | ID: mdl-33604858

RESUMEN

Various approaches have been evaluated for developing three-dimensional (3D) scaffolds for modeling or engineering of the bone tissue. However, most of such attempts have come up short in mimicking the natural bone tissue extracellular matrix (ECM) microenvironment, especially its natural bioactive content. Here we describe the methodology for the preparation of a natural ECM-based multichannel construct as a biomimetic 3D bone tissue model. We elucidate the construction of the composite scaffold incorporating decellularized small intestinal submucosa ECM, synthetic hydroxyapatite and poly(Îµ-caprolactone), and the mechanical stimulation of the cell-seeded construct under bioreactor culture.

Asunto(s)

Sustitutos de Huesos/química; Durapatita/química; Matriz Extracelular/química; Ingeniería de Tejidos/métodos; Andamios del Tejido/química; Animales; Materiales Biomiméticos/química; Biomimética/métodos; Matriz Ósea/química; Células Cultivadas; Humanos; Células Madre Mesenquimatosas/citología; Poliésteres/química; Impresión Tridimensional; Ratas

Palabras clave

3D bone tissue model; Biomimetic bone scaffold; Composite matrices; Decellularization; Extracellular matrix; Mechanical cue; Mesenchymal stem cell; Regenerative medicine

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Durapatita / Sustitutos de Huesos / Ingeniería de Tejidos / Matriz Extracelular / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos

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