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Proteome-wide and matrisome-specific alterations during human pancreas development and maturation.
Li, Zihui; Tremmel, Daniel M; Ma, Fengfei; Yu, Qinying; Ma, Min; Delafield, Daniel G; Shi, Yatao; Wang, Bin; Mitchell, Samantha A; Feeney, Austin K; Jain, Vansh S; Sackett, Sara Dutton; Odorico, Jon S; Li, Lingjun.
Afiliación
  • Li Z; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
  • Tremmel DM; Department of Surgery, Division of Transplantation, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Ma F; School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
  • Yu Q; School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
  • Ma M; School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
  • Delafield DG; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
  • Shi Y; School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
  • Wang B; School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
  • Mitchell SA; Department of Surgery, Division of Transplantation, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Feeney AK; Department of Surgery, Division of Transplantation, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Jain VS; Department of Surgery, Division of Transplantation, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Sackett SD; Department of Surgery, Division of Transplantation, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Odorico JS; Department of Surgery, Division of Transplantation, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA. jon@surgery.wisc.edu.
  • Li L; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA. lingjun.li@wisc.edu.
Nat Commun ; 12(1): 1020, 2021 02 15.
Article en En | MEDLINE | ID: mdl-33589611
The extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Páncreas / Proteínas de la Matriz Extracelular / Regulación del Desarrollo de la Expresión Génica / Proteoma Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Páncreas / Proteínas de la Matriz Extracelular / Regulación del Desarrollo de la Expresión Génica / Proteoma Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido