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Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation.
Du, Yongming; Yan, Yinxia; Xie, Si; Huang, Hao; Wang, Xin; Ng, Ray Kit; Zhou, Ming-Ming; Qian, Chengmin.
Afiliación
  • Du Y; School of Biomedical Sciences, The University of Hong Kong, Hong Kong Island, Hong Kong.
  • Yan Y; School of Biomedical Sciences, The University of Hong Kong, Hong Kong Island, Hong Kong.
  • Xie S; School of Biomedical Sciences, The University of Hong Kong, Hong Kong Island, Hong Kong.
  • Huang H; Department of Biomedical Sciences, The City University of Hong Kong, Kowloon, Hong Kong.
  • Wang X; Department of Biomedical Sciences, The City University of Hong Kong, Kowloon, Hong Kong.
  • Ng RK; School of Biomedical Sciences, The University of Hong Kong, Hong Kong Island, Hong Kong.
  • Zhou MM; Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Qian C; School of Biomedical Sciences, The University of Hong Kong, Hong Kong Island, Hong Kong. cmqian@hku.hk.
Nat Commun ; 12(1): 949, 2021 02 11.
Article en En | MEDLINE | ID: mdl-33574238
Spindlin1 is a unique multivalent epigenetic reader that facilitates ribosomal RNA transcription. In this study, we provide molecular and structural basis by which Spindlin1 acts in complex with C11orf84 to preferentially recognize non-canonical bivalent mark of trimethylated lysine 4 and lysine 9 present on the same histone H3 tail (H3K4me3K9me3). We demonstrate that C11orf84 binding stabilizes Spindlin1 and enhances its association with bivalent H3K4me3K9me3 mark. The functional analysis suggests that Spindlin1/C11orf84 complex can displace HP1 proteins from H3K4me3K9me3-enriched rDNA loci, thereby facilitating the conversion of these poised rDNA repeats from the repressed state to the active conformation, and the consequent recruitment of RNA Polymerase I for rRNA transcription. Our study uncovers a previously unappreciated mechanism of bivalent H3K4me3K9me3 recognition by Spindlin1/C11orf84 complex required for activation of rRNA transcription.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Histonas / Activación Transcripcional Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Histonas / Activación Transcripcional Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Reino Unido