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Comparison of species, virulence genes and clones of Aeromonas isolates from clinical specimens and well water in Okinawa Prefecture, Japan.
Miyagi, K; Shimoji, N; Shimoji, S; Tahara, R; Uechi, A; Tamaki, I; Oshiro, H; Komiyama, A; Tedokon, M; Hirai, I.
Afiliación
  • Miyagi K; Laboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
  • Shimoji N; Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
  • Shimoji S; Laboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
  • Tahara R; Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
  • Uechi A; Laboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
  • Tamaki I; Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
  • Oshiro H; Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan.
  • Komiyama A; Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
  • Tedokon M; Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
  • Hirai I; Department of Clinical Laboratory, Urasoe General Hospital, Okinawa, Japan.
J Appl Microbiol ; 131(3): 1515-1530, 2021 Sep.
Article en En | MEDLINE | ID: mdl-33570830
AIMS: To reveal the sources of Aeromonas infection in Okinawa Prefecture of Japan, the species, virulence genes and clones of strains isolated from clinical specimens and well water were compared. METHODS AND RESULTS: The properties of both isolates were investigated by sequencing of rpoD, detection of 10 virulence genes using PCR and genotyping with pulsed-field gel electrophoresis. In all, 68 clinical and 146 well water strains of Aeromonas were isolated and the main species were A. caviae, A. dhakensis, A. hydrophila and A. veronii biovar sobria. Aeromonas dhakensis possessed various virulence genes; however, A. caviae possessed only fla. The same or similar clones were distributed in certain areas of Okinawa and one clone had survived several months in the biliary system of two patients, respectively. CONCLUSION: Although the same Aeromonas clone was not isolated from clinical and well water samples, our study revealed the detected patterns of virulence genes in both isolates, the distribution of identical/similar clones in the Okinawan environment and long-time survival in patient's organs. SIGNIFICANCE AND IMPACT OF THE STUDY: We investigated the association between Aeromonas patients and well water exposure. This study provides the properties of species, virulence genes and clones of Aeromonas isolated from samples of these origins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virulencia / Agua Potable / Infecciones por Bacterias Gramnegativas / Aeromonas Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virulencia / Agua Potable / Infecciones por Bacterias Gramnegativas / Aeromonas Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido