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CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes.
Baker, Dave J; Aydin, Alp; Le-Viet, Thanh; Kay, Gemma L; Rudder, Steven; de Oliveira Martins, Leonardo; Tedim, Ana P; Kolyva, Anastasia; Diaz, Maria; Alikhan, Nabil-Fareed; Meadows, Lizzie; Bell, Andrew; Gutierrez, Ana Victoria; Trotter, Alexander J; Thomson, Nicholas M; Gilroy, Rachel; Griffith, Luke; Adriaenssens, Evelien M; Stanley, Rachael; Charles, Ian G; Elumogo, Ngozi; Wain, John; Prakash, Reenesh; Meader, Emma; Mather, Alison E; Webber, Mark A; Dervisevic, Samir; Page, Andrew J; O'Grady, Justin.
Afiliación
  • Baker DJ; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Aydin A; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Le-Viet T; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Kay GL; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Rudder S; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • de Oliveira Martins L; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Tedim AP; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Kolyva A; Grupo de Investigación Biomédica en Sepsis - BioSepsis. Hospital Universitario Rio Hortega/Instituto de Investigación Biomédica de Salamanca (IBSAL), Valladolid/Salamanca, Spain.
  • Diaz M; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Alikhan NF; Norfolk and Norwich University Hospital, Colney Lane, Norwich, NR4 7UY, UK.
  • Meadows L; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Bell A; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Gutierrez AV; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Trotter AJ; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Thomson NM; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Gilroy R; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Griffith L; University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • Adriaenssens EM; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Stanley R; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Charles IG; University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • Elumogo N; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Wain J; Norfolk and Norwich University Hospital, Colney Lane, Norwich, NR4 7UY, UK.
  • Prakash R; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Meader E; University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • Mather AE; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Webber MA; Norfolk and Norwich University Hospital, Colney Lane, Norwich, NR4 7UY, UK.
  • Dervisevic S; Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Page AJ; University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • O'Grady J; Norfolk and Norwich University Hospital, Colney Lane, Norwich, NR4 7UY, UK.
Genome Med ; 13(1): 21, 2021 02 09.
Article en En | MEDLINE | ID: mdl-33563320
We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced using all 3 methods, ≥ 90% genome coverage was obtained for 64.3% using ARTIC LoCost, 71.4% using CoronaHiT-ONT and 76.6% using CoronaHiT-Illumina, with almost identical clustering on a maximum likelihood tree. This protocol will aid the rapid expansion of SARS-CoV-2 genome sequencing globally.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma Viral / Pandemias / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: Genome Med Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma Viral / Pandemias / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: Genome Med Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido