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A 3D-printed microfluidic platform for simulating the effects of CPAP on the nasal epithelium.
Shrestha, Jesus; Ryan, Sean Thomas; Mills, Oliver; Zhand, Sareh; Razavi Bazaz, Sajad; Hansbro, Philip Michael; Ghadiri, Maliheh; Ebrahimi Warkiani, Majid.
Afiliación
  • Shrestha J; School of Biomedical Engineering, University of Technology Sydney, Sydney, New South Wales 2007, Australia.
  • Ryan ST; Woolcock Institute of Medical Research, Respiratory Technology Group, University of Sydney, Sydney, New South Wales 2037, Australia.
  • Mills O; Woolcock Institute of Medical Research, Respiratory Technology Group, University of Sydney, Sydney, New South Wales 2037, Australia.
  • Zhand S; Woolcock Institute of Medical Research, Respiratory Technology Group, University of Sydney, Sydney, New South Wales 2037, Australia.
  • Razavi Bazaz S; School of Biomedical Engineering, University of Technology Sydney, Sydney, New South Wales 2007, Australia.
  • Hansbro PM; School of Biomedical Engineering, University of Technology Sydney, Sydney, New South Wales 2007, Australia.
  • Ghadiri M; Centre for Inflammation, Centenary Institute and University of Technology Sydney, School of Life Sciences, Faculty of Science, Sydney, New South Wales 2050 and 2007, Australia.
  • Ebrahimi Warkiani M; School of Biomedical Engineering, University of Technology Sydney, Sydney, New South Wales 2007, Australia.
Biofabrication ; 13(3)2021 04 08.
Article en En | MEDLINE | ID: mdl-33561837
Obstructive sleep apnea (OSA) is a chronic disorder that involves a decrease or complete cessation of airflow during sleep. It occurs when the muscles supporting the soft tissues in the throat relax during sleep, causing narrowing or closure of the upper airway. Sleep apnea is a serious medical condition with an increased risk of cardiovascular complications and impaired quality of life. Continuous positive airway pressure (CPAP) is the most effective treatment for moderate to severe cases of OSA and is effective in mild sleep apnea. However, CPAP therapy is associated with the development of several nasal side effects and is inconvenient for the user, leading to low compliance rates. The effects of CPAP treatment on the upper respiratory system, as well as the pathogenesis of side effects, are incompletely understood and not adequately researched. To better understand the effects of CPAP treatment on the upper respiratory system, we developed anin vitro3D-printed microfluidic platform. A nasal epithelial cell line, RPMI 2650, was then exposed to certain conditions to mimic thein vivoenvironment. To create these conditions, the microfluidic device was utilized to expose nasal epithelial cells grown and differentiated at the air-liquid interface. The airflow was similar to what is experienced with CPAP, with pressure ranging between 0 and 20 cm of H2O. Cells exposed to pressure showed decreased barrier integrity, change in cellular shape, and increased cell death (lactate dehydrogenase release into media) compared to unstressed cells. Stressed cells also showed increased secretions of inflammatory markers IL-6 and IL-8 and had increased production of ATP. Our results suggest that stress induced by airflow leads to structural, metabolic, and inflammatory changes in the nasal epithelium, which may be responsible for developing nasal side-effects following CPAP treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calidad de Vida / Presión de las Vías Aéreas Positiva Contínua Aspecto: Patient_preference Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calidad de Vida / Presión de las Vías Aéreas Positiva Contínua Aspecto: Patient_preference Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido