Your browser doesn't support javascript.
loading
Diazepam's antifungal activity in fluconazole-resistant Candida spp. and biofilm inhibition in C. albicans: evaluation of the relationship with the proteins ALS3 and SAP5.
Juvêncio da Silva, Lisandra; Dias Barroso, Fátima Daiana; Vieira, Lucas Sousa; Carlos Mota, Daniel Roberto; da Silva Firmino, Bruna Kelly; Rocha da Silva, Cecília; de Farias Cabral, Vitória Pessoa; Cândido, Thiago Mesquita; Sá, Lívia Gurgel do Amaral Valente; Barbosa da Silva, Wildson Max; Silva, Jacilene; Marinho, Emmanuel Silva; Cavalcanti, Bruno Coelho; de Moraes, Manoel Odorico; Júnior, Hélio Vitoriano Nobre; de Andrade Neto, João Batista.
Afiliación
  • Juvêncio da Silva L; Drug Research and Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
  • Dias Barroso FD; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
  • Vieira LS; Drug Research and Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
  • Carlos Mota DR; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
  • da Silva Firmino BK; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil.
  • Rocha da Silva C; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil.
  • de Farias Cabral VP; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil.
  • Cândido TM; Drug Research and Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
  • Sá LGDAV; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
  • Barbosa da Silva WM; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
  • Silva J; Drug Research and Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
  • Marinho ES; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
  • Cavalcanti BC; Drug Research and Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
  • de Moraes MO; School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
  • Júnior HVN; Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil.
  • de Andrade Neto JB; Department of Chemistry, Group for Theoretical Chemistry and Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, Brazil.
J Med Microbiol ; 70(3)2021 Mar.
Article en En | MEDLINE | ID: mdl-33560202
The genus Candida spp. has been highlighted as one of the main etiological agents causing fungal infections, with Candida albicans being the most prominent, responsible for most cases of candidemia. Due to its capacity for invasion and tissue adhesion, it is associated with the formation of biofilms, mainly in the environment and hospital devices, decreasing the effectiveness of available treatments. The repositioning of drugs, which is characterized by the use of drugs already on the market for other purposes, together with molecular-docking methods can be used aiming at the faster development of new antifungals to combat micro-organisms. This study aimed to evaluate the antifungal effect of diazepam on mature C. albicans biofilms in vitro and its action on biofilm in formation, as well as its mechanism of action and interaction with structures related to the adhesion of C. albicans, ALS3 and SAP5. To determine the MIC, the broth microdilution test was used according to protocol M27-A3 (CLSI, 2008). In vitro biofilm formation tests were performed using 96-well plates, followed by molecular-docking protocols to analyse the binding agent interaction with ALS3 and SAP5 targets. The results indicate that diazepam has antimicrobial activity against planktonic cells of Candida spp. and C. albicans biofilms, interacting with important virulence factors related to biofilm formation (ALS3 and SAP5). In addition, treatment with diazepam triggered a series of events in C. albicans cells, such as loss of membrane integrity, mitochondrial depolarization and increased production of EROs, causing DNA damage and consequent cell apoptosis.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Biopelículas / Farmacorresistencia Fúngica / Diazepam / Antifúngicos Tipo de estudio: Guideline Idioma: En Revista: J Med Microbiol Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Biopelículas / Farmacorresistencia Fúngica / Diazepam / Antifúngicos Tipo de estudio: Guideline Idioma: En Revista: J Med Microbiol Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido