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Synthetic polymers as xeno-free materials for stabilizing basic fibroblast growth factor in human mesenchymal stem cell cultures.
Masuzawa, Yoko; Kitazawa, Manabu.
Afiliación
  • Masuzawa Y; Institute for Innovation, Ajinomoto Co. Inc, 1-1 Suzuki-Cho, Kawasaki-Ku, Kawasaki City, Kanagawa, Japan.
  • Kitazawa M; Institute for Innovation, Ajinomoto Co. Inc, 1-1 Suzuki-Cho, Kawasaki-Ku, Kawasaki City, Kanagawa, Japan.
Biochem Biophys Rep ; 25: 100929, 2021 Mar.
Article en En | MEDLINE | ID: mdl-33553689
Series of sulfonated polymers were evaluated as additives in cell culture media. Some of the compounds, such as sulfated polyvinyl alcohol (PVA), prevented denaturation and loss of basic fibroblast growth factor during cell culture and enhanced human mesenchymal stem cell proliferation. These compounds are xeno-free alternatives of heparin, an animal-derived sulfated saccharide, often used as an additive. To the best our knowledge, this study is the first to show that chemically defined synthetic chemicals, such as sulfated polyvinyl alcohol, can be used for this purpose.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biochem Biophys Rep Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biochem Biophys Rep Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos