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Magnetic Nanoparticles to Unique DNA Tracers: Effect of Functionalization on Physico-chemical Properties.
Sharma, Anuvansh; Foppen, Jan Willem; Banerjee, Abhishek; Sawssen, Slimani; Bachhar, Nirmalya; Peddis, Davide; Bandyopadhyay, Sulalit.
Afiliación
  • Sharma A; Department of Materials Science and Engineering, Norwegian University of Science and Technology (NTNU), 7491, Trondheim, Norway.
  • Foppen JW; Department of Water Science and Engineering, IHE Delft Institute for Water Education, PO Box 3015, Delft, The Netherlands.
  • Banerjee A; Department of Materials Science and Engineering, Norwegian University of Science and Technology (NTNU), 7491, Trondheim, Norway.
  • Sawssen S; Dipartimento di Chimica e Chimica Industriale, Università di Genova, Genoa, Italy.
  • Bachhar N; Istituto di Struttura della Materia - CNR, Area della Ricerca di Roma1, 00015, Monterotondo Scalo, RM, Italy.
  • Peddis D; Department of Chemical Engineering, Indian Institute of Technology Jodhpur, Jodhpur, 342037, India.
  • Bandyopadhyay S; Dipartimento di Chimica e Chimica Industriale, Università di Genova, Genoa, Italy.
Nanoscale Res Lett ; 16(1): 24, 2021 Feb 06.
Article en En | MEDLINE | ID: mdl-33547989
To monitor and manage hydrological systems such as brooks, streams, rivers, the use of tracers is a well-established process. Limited number of potential tracers such as salts, isotopes and dyes, make study of hydrological processes a challenge. Traditional tracers find limited use due to lack of multiplexed, multipoint tracing and background noise, among others. In this regard, DNA based tracers possess remarkable advantages including, environmentally friendly, stability, and high sensitivity in addition to showing great potential in the synthesis of ideally unlimited number of unique tracers capable of multipoint tracing. To prevent unintentional losses in the environment during application and easy recovery for analysis, we hereby report DNA encapsulation in silica containing magnetic cores (iron oxide) of two different shapes-spheres and cubes. The iron oxide nanoparticles having size range 10-20 nm, have been synthesized using co-precipitation of iron salts or thermal decomposition of iron oleate precursor in the presence of oleic acid or sodium oleate. Physico-chemical properties such as size, zeta potential, magnetism etc. of the iron oxide nanoparticles have been optimized using different ligands for effective binding of dsDNA, followed by silanization. We report for the first time the effect of surface coating on the magnetic properties of the iron oxide nanoparticles at each stage of functionalization, culminating in silica shells. Efficiency of encapsulation of three different dsDNA molecules has been studied using quantitative polymerase chain reaction (qPCR). Our results show that our DNA based magnetic tracers are excellent candidates for hydrological monitoring with easy recoverability and high signal amplification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nanoscale Res Lett Año: 2021 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nanoscale Res Lett Año: 2021 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Estados Unidos