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Perfusion Flow Enhances Viability and Migratory Phenotype in 3D-Cultured Breast Cancer Cells.
Pasini, Alice; Lovecchio, Joseph; Cortesi, Marilisa; Liverani, Chiara; Spadazzi, Chiara; Mercatali, Laura; Ibrahim, Toni; Giordano, Emanuele.
Afiliación
  • Pasini A; Laboratory of Cellular and Molecular Engineering "Silvio Cavalcanti", Department of Electrical, Electronic and Information Engineering "G. Marconi" (DEI), Alma Mater Studiorum - University of Bologna, Cesena, FC, Italy.
  • Lovecchio J; Laboratory of Cellular and Molecular Engineering "Silvio Cavalcanti", Department of Electrical, Electronic and Information Engineering "G. Marconi" (DEI), Alma Mater Studiorum - University of Bologna, Cesena, FC, Italy. joseph.lovecchio@unibo.it.
  • Cortesi M; Laboratory of Cellular and Molecular Engineering "Silvio Cavalcanti", Department of Electrical, Electronic and Information Engineering "G. Marconi" (DEI), Alma Mater Studiorum - University of Bologna, Cesena, FC, Italy.
  • Liverani C; Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
  • Spadazzi C; Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
  • Mercatali L; Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
  • Ibrahim T; Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
  • Giordano E; Laboratory of Cellular and Molecular Engineering "Silvio Cavalcanti", Department of Electrical, Electronic and Information Engineering "G. Marconi" (DEI), Alma Mater Studiorum - University of Bologna, Cesena, FC, Italy.
Ann Biomed Eng ; 49(9): 2103-2113, 2021 Sep.
Article en En | MEDLINE | ID: mdl-33543395
Conventional 2D cell culture, a traditional tool in pre-clinical studies, can hardly be regarded as a representation of a natural cell microenvironment. In this respect, it might result in altered cellular behaviors. To overcome such a limitation, different approaches have been tested to conduct more representative in vitro studies. In particular, the use of 3D cell culture introduces variables, such as cell-cell and cell-extracellular matrix interactions; cell features such as survival, proliferation and migration are consequently influenced. For an example, an enhanced drug resistance and increased invasiveness are shown by cancer cells when cultured in 3D versus 2D conventional culture models. In this setting however, non-uniform cell distribution and biological behaviors appear throughout the scaffold, due to reduced diffusion of oxygen and nutrients. Perfusion in bioreactor systems can be used to improve medium transport. In this line of reasoning, this study proposes a breast cancer cell culture model sustained by an integrated approach that couples a 3D environment and a fluid perfusion. This model improves viability and uniformness of cell distribution, while inducing morphological, functional and molecular cancer cell remodeling.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Reactores Biológicos Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Ann Biomed Eng Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Reactores Biológicos Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Ann Biomed Eng Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos