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Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.
Gharanei, Seley; Fishwick, Katherine; Peter Durairaj, Ruban; Jin, Tianrong; Siamantouras, Eleftherios; Liu, Kuo-Kang; Straube, Anne; Lucas, Emma S; Weston, Christopher J; Rantakari, Pia; Salmi, Marko; Jalkanen, Sirpa; Brosens, Jan J; Tan, Bee Kang.
Afiliación
  • Gharanei S; Warwick Medical School, University of Warwick, Coventry, United Kingdom.
  • Fishwick K; Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism, University Hospitals Coventry and Warwickshire National Health Service Trust, Coventry, United Kingdom.
  • Peter Durairaj R; Warwick Medical School, University of Warwick, Coventry, United Kingdom.
  • Jin T; Warwick Medical School, University of Warwick, Coventry, United Kingdom.
  • Siamantouras E; School of Engineering, University of Warwick, Coventry, United Kingdom.
  • Liu KK; School of Engineering, University of Warwick, Coventry, United Kingdom.
  • Straube A; School of Engineering, University of Warwick, Coventry, United Kingdom.
  • Lucas ES; Warwick Medical School, University of Warwick, Coventry, United Kingdom.
  • Weston CJ; Centre for Mechanochemical Cell Biology, University of Warwick, Coventry, United Kingdom.
  • Rantakari P; Warwick Medical School, University of Warwick, Coventry, United Kingdom.
  • Salmi M; Tommy's National Centre for Miscarriage Research, Coventry, United Kingdom.
  • Jalkanen S; Centre for Liver Research & National Institute for Health Research Birmingham Biomedical Research Unit, Level 5 Institute for Biomedical Research, University of Birmingham, Birmingham, United Kingdom.
  • Brosens JJ; Medicity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland.
  • Tan BK; Medicity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland.
Front Cell Dev Biol ; 8: 621016, 2020.
Article en En | MEDLINE | ID: mdl-33537312
Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible adhesion molecule and a primary amine oxidase involved in immune cell trafficking. Leukocyte extravasation into tissues is mediated by adhesion molecules expressed on endothelial cells and pericytes. Pericytes play a major role in the angiogenesis and vascularization of cycling endometrium. However, the functional properties of pericytes in the human endometrium are not known. Here we show that pericytes surrounding the spiral arterioles in midluteal human endometrium constitutively express VAP-1. We first characterize these pericytes and demonstrate that knockdown of VAP-1 perturbed their biophysical properties and compromised their contractile, migratory, adhesive and clonogenic capacities. Furthermore, we show that loss of VAP-1 disrupts pericyte-uterine natural killer cell interactions in vitro. Taken together, the data not only reveal that endometrial pericytes represent a cell population with distinct biophysical and functional properties but also suggest a pivotal role for VAP-1 in regulating the recruitment of innate immune cells in human endometrium. We posit that VAP-1 could serve as a potential biomarker for pregnancy pathologies caused by a compromised perivascular environment prior to conception.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Dev Biol Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Dev Biol Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza