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Degradome sequencing-based identification of phasiRNAs biogenesis pathways in Oryza sativa.
Yu, Lan; Guo, Rongkai; Jiang, Yeqin; Ye, Xinghuo; Yang, Zhihong; Meng, Yijun; Shao, Chaogang.
Afiliación
  • Yu L; College of Life Sciences, Huzhou University, NO.1 Xueshi Road, Huzhou, 313000, P.R. China.
  • Guo R; Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
  • Jiang Y; College of Life Sciences, Huzhou University, NO.1 Xueshi Road, Huzhou, 313000, P.R. China.
  • Ye X; College of Life Sciences, Huzhou University, NO.1 Xueshi Road, Huzhou, 313000, P.R. China.
  • Yang Z; College of Life Sciences, Huzhou University, NO.1 Xueshi Road, Huzhou, 313000, P.R. China.
  • Meng Y; College of Life and Environmental Sciences, Hangzhou Normal University, Xuelin Street 16#, Xiasha, Hangzhou, 310036, P. R. China. mengyijun@zju.edu.cn.
  • Shao C; College of Life Sciences, Huzhou University, NO.1 Xueshi Road, Huzhou, 313000, P.R. China. shaocg@zjhu.edu.cn.
BMC Genomics ; 22(1): 93, 2021 Jan 30.
Article en En | MEDLINE | ID: mdl-33516199
BACKGROUND: The microRNAs(miRNA)-derived secondary phased small interfering RNAs (phasiRNAs) participate in post-transcriptional gene silencing and play important roles in various bio-processes in plants. In rice, two miRNAs, miR2118 and miR2275, were mainly responsible for triggering of 21-nt and 24-nt phasiRNAs biogenesis, respectively. However, relative fewer phasiRNA biogenesis pathways have been discovered in rice compared to other plant species, which limits the comprehensive understanding of phasiRNA biogenesis and the miRNA-derived regulatory network. RESULTS: In this study, we performed a systematical searching for phasiRNA biogenesis pathways in rice. As a result, five novel 21-nt phasiRNA biogenesis pathways and five novel 24-nt phasiRNA biogenesis pathways were identified. Further investigation of their regulatory function revealed that eleven novel phasiRNAs in 21-nt length recognized forty-one target genes. Most of these genes were involved in the growth and development of rice. In addition, five novel 24-nt phasiRNAs targeted to the promoter of an OsCKI1 gene and thereafter resulted in higher level of methylation in panicle, which implied their regulatory function in transcription of OsCKI1,which acted as a regulator of rice development. CONCLUSIONS: These results substantially extended the information of phasiRNA biogenesis pathways and their regulatory function in rice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / MicroARNs Tipo de estudio: Diagnostic_studies Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / MicroARNs Tipo de estudio: Diagnostic_studies Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido