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Cell-Laden Gradient Hydrogel Scaffolds for Neovascularization of Engineered Tissues.
He, Yusheng J; Santana, Martin F; Staneviciute, Austeja; Pimentel, Marja B; Yang, Feipeng; Goes, Jacob; Kawaji, Keigo; Vaicik, Marcella K; Abdulhadi, Rayan; Hibino, Narutoshi; Papavasiliou, Georgia.
Afiliación
  • He YJ; Department of Surgery, University of Chicago, 5841 S Maryland Ave, Suit E500, Chicago, IL, 60637, USA.
  • Santana MF; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Staneviciute A; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Pimentel MB; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Yang F; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Goes J; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Kawaji K; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Vaicik MK; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Abdulhadi R; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
  • Hibino N; Department of Surgery, University of Chicago, 5841 S Maryland Ave, Suit E500, Chicago, IL, 60637, USA.
  • Papavasiliou G; Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Suite 314, Chicago, IL, 60616, USA.
Adv Healthc Mater ; 10(7): e2001706, 2021 04.
Article en En | MEDLINE | ID: mdl-33511790
Gradients in mechanical properties, physical architecture and biochemical composition exist in a variety of complex tissues, yet 3D in vitro models that enable investigation of these cues on cellular processes, especially those contributing to vascularization of engineered tissues are limited. Here, a photopolymerization approach to create cell-laden hydrogel biomaterials with decoupled and combined gradients in modulus, immobilized cell adhesive peptide (RGD) concentration, and proteolytic degradation enabling spatial encapsulation of vascular spheroids is reported to elucidate their impact on vascular sprouting in 3D culture. Vascular spheroids encapsulated in these gradient scaffolds exhibit spatial variations in total sprout length. Scaffolds presenting an immobilized RGD gradient promote biased vascular sprouting toward increasing RGD concentration. Importantly, biased sprouting is found to be dependent on immobilized RGD gradient characteristics, including magnitude and slope, with increases in these factors contributing to significant enhancements in biased sprouting responses. Conversely, reduction in biased sprouting responses is observed in combined gradient scaffolds possessing opposing gradients in RGD and modulus. The presented work is the first to demonstrate the use of a cell-laden biomaterial platform to systematically investigate the role of multiple scaffold gradients as well as gradient slope, magnitude and orientation on vascular sprouting responses in 3D culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Hidrogeles Tipo de estudio: Prognostic_studies Idioma: En Revista: Adv Healthc Mater Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Hidrogeles Tipo de estudio: Prognostic_studies Idioma: En Revista: Adv Healthc Mater Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania