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GC-MS/MS analysis of metabolites derived from a single human blastocyst.
Inoue, Naomi; Nishida, Yoshihiro; Harada, Emi; Sakai, Kumiko; Narahara, Hisashi.
Afiliación
  • Inoue N; Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
  • Nishida Y; Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan. ynishida@oita-u.ac.jp.
  • Harada E; Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
  • Sakai K; Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
  • Narahara H; Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
Metabolomics ; 17(2): 17, 2021 01 25.
Article en En | MEDLINE | ID: mdl-33495963
INTRODUCTION: The field of assisted reproductive technology (ART) has significantly advanced; however, morphological evaluation remains as the chosen method of assessment of embryo quality. OBJECTIVE: We aimed to examine metabolic changes in embryo culture medium to develop a non-invasive method for evaluation of embryo quality. METHODS: We performed metabolic analysis of culture medium obtained from a single blastocyst cultured for freezing. RESULTS: In total, 187 (39.8%) of the 469 detectable organic acid metabolites were identified. A significant change (p < 0.05) was observed in eight metabolites between the good-quality and poor-quality embryo groups. Differences were observed in several metabolic pathways between the good-quality and poor-quality embryo groups. Metabolites that showed significant changes were primarily involved in the metabolism of branched-chain amino acids. CONCLUSION: The quantification of metabolism in human embryos may assist in identification and selection of good-quality embryos with high rates of survival before freezing and implantation in conjunction with morphological classification. This may help to identify embryos with high rates of survival.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Blastocisto / Metabolómica / Cromatografía de Gases y Espectrometría de Masas Límite: Female / Humans / Male Idioma: En Revista: Metabolomics Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Blastocisto / Metabolómica / Cromatografía de Gases y Espectrometría de Masas Límite: Female / Humans / Male Idioma: En Revista: Metabolomics Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos