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Elastin-like hydrogel stimulates angiogenesis in a severe model of critical limb ischemia (CLI): An insight into the glyco-host response.
Marsico, Grazia; Jin, Chunseng; Abbah, Sunny A; Brauchle, Eva M; Thomas, Dilip; Rebelo, Ana Lúcia; Orbanic, Doriana; Chantepie, Sandrine; Contessotto, Paolo; Papy-Garcia, Dulce; Rodriguez-Cabello, Carlos; Kilcoyne, Michelle; Schenke-Layland, K; Karlsson, N G; McCullagh, Karl J A; Pandit, Abhay.
Afiliación
  • Marsico G; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Jin C; Department of Medical Biochemistry and Cell Biology at Institute of Biomedicine, Sahlgrenska Academy, The University of Gothenburg, Sweden.
  • Abbah SA; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Brauchle EM; Department of Women's Health, Research Institute for Women's Health, The Eberhard-Karls-University Tuebingen, Germany; The Natural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, Germany.
  • Thomas D; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Rebelo AL; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Orbanic D; Bioforge, Ciber-BBN, The University of Valladolid, Spain.
  • Chantepie S; Cell Growth, Tissue Repair and Regeneration (CRRET), UPEC EA 4397/ERL CNRS 9215, Université Paris Est Créteil, Université Paris Est, Créteil, France.
  • Contessotto P; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Papy-Garcia D; Cell Growth, Tissue Repair and Regeneration (CRRET), UPEC EA 4397/ERL CNRS 9215, Université Paris Est Créteil, Université Paris Est, Créteil, France.
  • Rodriguez-Cabello C; Bioforge, Ciber-BBN, The University of Valladolid, Spain.
  • Kilcoyne M; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland; Carbohydrate Signalling Group, Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Schenke-Layland K; Department of Women's Health, Research Institute for Women's Health, The Eberhard-Karls-University Tuebingen, Germany; The Natural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, Germany.
  • Karlsson NG; Department of Medical Biochemistry and Cell Biology at Institute of Biomedicine, Sahlgrenska Academy, The University of Gothenburg, Sweden.
  • McCullagh KJA; Physiology Department, National University of Ireland Galway, Galway H92 W2TY, Ireland.
  • Pandit A; CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland. Electronic address: abhay.pandit@nuigalway.ie.
Biomaterials ; 269: 120641, 2021 02.
Article en En | MEDLINE | ID: mdl-33493768
Critical limb ischemia (CLI) is characterized by the impairment of microcirculation, necrosis and inflammation of the muscular tissue. Although the role of glycans in mediating inflammation has been reported, changes in the glycosylation following muscle ischemia remains poorly understood. Here, a murine CLI model was used to show the increase of high mannose, α-(2, 6)-sialic acid and the decrease of hybrid and bisected N-glycans as glycosylation associated with the ischemic environment. Using this model, the efficacy of an elastin-like recombinamers (ELR) hydrogel was assessed. The hydrogel modulates key angiogenic signaling pathways, resulting in capillary formation, and ECM remodeling. Arterioles formation, reduction of fibrosis and anti-inflammatory macrophage polarization wa also induced by the hydrogel administration. Modulation of glycosylation was observed, suggesting, in particular, a role for mannosylation and sialylation in the mediation of tissue repair. Our study elucidates the angiogenic potential of the ELR hydrogel for CLI applications and identifies glycosylation alterations as potential new therapeutic targets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elastina / Neovascularización Fisiológica / Hidrogeles / Isquemia Límite: Animals Idioma: En Revista: Biomaterials Año: 2021 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elastina / Neovascularización Fisiológica / Hidrogeles / Isquemia Límite: Animals Idioma: En Revista: Biomaterials Año: 2021 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Países Bajos