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Characterization and Detection of Passiflora Mottle Virus and Two Other Potyviruses Causing Passionfruit Woodiness Disease in Vietnam.
Do, Duy-Hung; Chong, Yee-Hang; Ha, Viet-Cuong; Cheng, Hao-Wen; Chen, Yuh-Kun; Bui, Thi-Ngoc-Lan; Nguyen, Thi-Bich-Ngoc; Yeh, Shyi-Dong.
Afiliación
  • Do DH; Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
  • Chong YH; Plant Pathology Division, Plant Protection Research Institute, Hanoi, Vietnam.
  • Ha VC; Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
  • Cheng HW; Overseas Vietnam Agricultural Science and Technology Innovation Center, National Chung Hsing University, Taichung, Taiwan, R.O.C.
  • Chen YK; Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi, Vietnam.
  • Bui TN; Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
  • Nguyen TB; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, R.O.C.
  • Yeh SD; Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Phytopathology ; 111(9): 1675-1685, 2021 Sep.
Article en En | MEDLINE | ID: mdl-33487021
Passionfruit plantings in Vietnam increased to 10,000 ha in 2019. However, outbreaks of passionfruit woodiness disease (PWD) have become a serious threat to production. In this study, five virus isolates (DN1, DN4, NA1, GL1, and GL2) were collected from different areas of Vietnam. Their causal roles in PWD were verified by back-inoculation to passionfruit. Analyses of coat protein (CP) and genomic sequences revealed that the GL1 isolate is closely related to East Asia Passiflora virus (EAPV) AO strain of Japan (polyprotein nt and aa identities of 98.1 and 98.2%, respectively), and the GL2 isolate is related to Telosma mosaic virus (TelMV) isolate PasFru, China (polyprotein nt and aa identities of 87.1 and 90.9%, respectively). CP comparison, host range, and cytological characterization indicated that DN1, DN4, and NA1 are potyviruses but are different from EAPV and TelMV. Phylogenic analyses of their CP and genome sequences indicated that these three isolates and the passionfruit severe mottle-associated virus Fujian isolate of China belong to a distinct clade, which does not meet the threshold (76% nt identity of polyprotein) to be regarded as any of potyviral species. Thus, a new species name, Passiflora mottle virus, (PaMoV), has been proposed by the International Committee on Taxonomy of Viruses. A rabbit antiserum was produced against the CP of DN1, and it can distinguish PaMoV from TelMV and EAPV in western blotting and enzyme-linked immunosorbent assay (ELISA) without cross-reactions. Field surveys of 240 samples by ELISA and reverse transcription PCR found that PWD in Vietnam is caused mainly by PaMoV, followed by EAPV, mixed infection of PaMoV and EAPV, and rare cases of TelMV.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Potyvirus / Passiflora Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Potyvirus / Passiflora Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos