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Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
Sanman, Laura E; Chen, Ina W; Bieber, Jake M; Steri, Veronica; Trentesaux, Coralie; Hann, Byron; Klein, Ophir D; Wu, Lani F; Altschuler, Steven J.
Afiliación
  • Sanman LE; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Chen IW; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Bieber JM; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA; Graduate Program in Bioengineering, University of California, San Francisco and University of California, Berkeley, San Francisco, CA 94158, USA.
  • Steri V; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94158, USA; Preclinical Therapeutics Core, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Trentesaux C; Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Hann B; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94158, USA; Preclinical Therapeutics Core, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Klein OD; Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Pediatrics and Institute for Human Genetics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Wu LF; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: lani.wu@ucsf.edu.
  • Altschuler SJ; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: steven.altschuler@ucsf.edu.
Dev Cell ; 56(3): 356-365.e9, 2021 02 08.
Article en En | MEDLINE | ID: mdl-33484640
Renewing tissues have the remarkable ability to continually produce both proliferative progenitor and specialized differentiated cell types. How are complex milieus of microenvironmental signals interpreted to coordinate tissue-cell-type composition? Here, we investigate the responses of intestinal epithelium to individual and paired perturbations across eight epithelial signaling pathways. Using a high-throughput approach that combines enteroid monolayers and quantitative imaging, we identified conditions that enrich for specific cell types as well as interactions between pathways. Importantly, we found that modulation of transit-amplifying cell proliferation changes the ratio of differentiated secretory to absorptive cell types. These observations highlight an underappreciated role for transit-amplifying cells in the tuning of differentiated cell-type composition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Epiteliales / Intestinos Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Epiteliales / Intestinos Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos