Your browser doesn't support javascript.
loading
Visualization of the Oncolytic Alphavirus M1 Life Cycle in Cancer Cells.
Dan, Jia; Nie, Lin; Jia, Xudong; Xu, Cuiying; Cai, Jing; Lin, Yuan; Hu, Jun; Zhu, Wenbo; Li, Yinyin; Chen, Dong; Liu, Ying; Hu, Cheng; Yan, Guangmei; Liang, Jiankai; Zhang, Qinfen.
Afiliación
  • Dan J; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Nie L; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
  • Jia X; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
  • Xu C; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Cai J; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Lin Y; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Hu J; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Zhu W; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Li Y; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
  • Chen D; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
  • Liu Y; Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.
  • Hu C; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.
  • Yan G; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Liang J; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China. liangjk5@mail.sysu.edu.cn.
  • Zhang Q; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. lsszqf@mail.sysu.edu.cn.
Virol Sin ; 36(4): 655-666, 2021 Aug.
Article en En | MEDLINE | ID: mdl-33481190
Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells, but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear. Here, we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell line by transmission electron microscopy, specifically examining viral entry, assembly, maturation and release. We found that M1 virus induces vacuolization of cancer cells during infection and ultimately nuclear marginalization, a typical indicator of apoptosis. Specifically, our results suggest that the endoplasmic reticulum participates in the assembly of nucleocapsids. In the early and late stage of infection, three kinds of special cytopathic vacuoles are formed and appear to be involved in the replication, maturation and release of the virus. Taken together, our data displayed the process of M1 virus infection of tumor cells and provide the structural basis for the study of M1 virus-host interactions.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alphavirus / Virus Oncolíticos / Viroterapia Oncolítica / Neoplasias Límite: Animals Idioma: En Revista: Virol Sin Asunto de la revista: VIROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alphavirus / Virus Oncolíticos / Viroterapia Oncolítica / Neoplasias Límite: Animals Idioma: En Revista: Virol Sin Asunto de la revista: VIROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos