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Development of an in vitro gastro-intestinal pig model to screen potential detoxifying agents for the mycotoxin deoxynivalenol.
Vanhoutte, Ilse; Vande Ginste, Jan; Verstringe, Stefanie; Vidal, Arnau; De Boevre, Marthe; De Saeger, Sarah; Audenaert, Kris; De Gelder, Leen.
Afiliación
  • Vanhoutte I; Laboratory of Environmental Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
  • Vande Ginste J; Nutrition Sciences N.V., Drongen, Belgium.
  • Verstringe S; Nutrition Sciences N.V., Drongen, Belgium.
  • Vidal A; Centre of Excellence in Mycotoxicology and Public Health, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  • De Boevre M; Centre of Excellence in Mycotoxicology and Public Health, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  • De Saeger S; Centre of Excellence in Mycotoxicology and Public Health, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  • Audenaert K; Laboratory of Applied Mycology and Phenomics, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
  • De Gelder L; Laboratory of Environmental Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess ; 38(3): 488-500, 2021 Mar.
Article en En | MEDLINE | ID: mdl-33480829
Deoxynivalenol (DON) is a type B trichothecene mycotoxin with worldwide high incidence in feed which is produced by Fusarium species. Strategies are needed to eliminate its health risk for livestock and to minimise its economic impact on production. In order to assess the efficacy of potential physical, chemical and biological DON detoxifying agents, a good in vitro model is necessary to perform a fast and high-throughput screening of new compounds before in vivo trials are set up. In this paper, an in vitro model was developed to screen potential commercial products for DON degradation and detoxification. Contaminated feed with potential detoxifying agents are first applied to a simulated gastrointestinal tract (GIT) of a pig, after which detoxification is assessed through a robust, inexpensive and readily applicable Lemna minor L. aquatic plant bioassay which enables evaluation of the residual toxicity of possible metabolites formed by DON detoxifying agents. The GIT simulation enables taking matrix and incubation parameters into account as they can affect the binding, removal or degradation of DON. One product could reduce DON in feed in the GIT model for almost 100% after 6 h. DON metabolites were tentatively identified with LC-MS/MS. This GIT simulation coupled to a detoxification bioassay is a valuable model for in vitro screening and assessing compounds for DON detoxification, and could be expanded towards other mycotoxins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tricotecenos / Tracto Gastrointestinal / Alimentación Animal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2021 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tricotecenos / Tracto Gastrointestinal / Alimentación Animal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2021 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido