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Deficiency of the RIß subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats.
Hoang Trung, Hieu; Yoshihara, Toru; Nakao, Akito; Hayashida, Katsumi; Hirata, Yoshiki; Shirasuna, Koumei; Kuwamura, Mitsuru; Nakagawa, Yuki; Kaneko, Takehito; Mori, Yasuo; Asano, Masahide; Kuramoto, Takashi.
Afiliación
  • Hoang Trung H; Laboratory of Animal Nutrition, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan.
  • Yoshihara T; Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan.
  • Nakao A; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 615-8510, Japan.
  • Hayashida K; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 615-8510, Japan.
  • Hirata Y; Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan.
  • Shirasuna K; Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan.
  • Kuwamura M; Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Science, Osaka Prefecture University, 1-58 Rinkuuourai-kita, Izumisano, Osaka, 598-8531, Japan.
  • Nakagawa Y; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
  • Kaneko T; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
  • Mori Y; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 615-8510, Japan.
  • Asano M; Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan.
  • Kuramoto T; Laboratory of Animal Nutrition, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan. tk206782@nodai.ac.jp.
Sci Rep ; 11(1): 2039, 2021 01 21.
Article en En | MEDLINE | ID: mdl-33479380
The RIß subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIß subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIß subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC-furue, a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC-furue genome. Prkar1b-deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral-CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIß subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIß function.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Temblor / Subunidad RIbeta de la Proteína Quinasa Dependiente de AMP Cíclico / Hipocampo / Trastornos de la Memoria Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Temblor / Subunidad RIbeta de la Proteína Quinasa Dependiente de AMP Cíclico / Hipocampo / Trastornos de la Memoria Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido