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Correlations between Resistance Classifications Based on Penicillin-Binding Protein Genotypes and Antimicrobial Susceptibility Test Results of Haemophilus influenzae.
Funashima, Yumiko; Nagasawa, Yoshihiro; Narita, Taeko; Hanaiwa, Hiroki; Nagasawa, Zenzo.
Afiliación
  • Funashima Y; Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare. Medical Laboratory Science, Graduate School of Health and Welfare Sciences, International University of Health and Welfare.
  • Nagasawa Y; Medical Departments, Self Defense Force Kumamoto Hospital.
  • Narita T; Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.
  • Hanaiwa H; Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.
  • Nagasawa Z; Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare. Medical Laboratory Science, Graduate School of Health and Welfare Sciences, International University of Health and Welfare. Department of Clinical Laboratory, Medical Kouhouk
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi ; 30(1): 7-15, 2020 Dec 25.
Article en En | MEDLINE | ID: mdl-33478232
There are several problems associated with antimicrobial susceptibility testing (AST) of Haemophilus influenzae. ß-Lactamase-negative ampicillin-resistant H. influenzae (BLNAR) isolates with minimum inhibitory concentration (MIC) of ampicillin (ABPC) <4 mg/l will be classified as susceptible according to the MIC breakpoint of the CLSI M100 criteria, in spite of harboring penicillin-binding protein (PBP) mutations that cause ABPC resistance. A total of 103 isolates were collected from clinical materials for analysis. The genotypes of the PBP mutations were analyzed by polymerase chain reaction. The WalkAway 96 Plus (WALKAWAY), dry plate Eiken (DP-EIKEN), and RAISUS S4 systems (RAISUS) were used for AST. HTM broth was used as the culture medium for WALKAWAY, Mueller‒Hinton broth with 5% lysed horse blood for DP-EIKEN, and HTM with 5% horse serum for RAISUS. The MIC concordance rates of ABPC for g-BLNAR, for RAISUS vs. DP-EIKEN, RAISUS vs. WALKAWAY, and DP-EIKEN vs. WALKAWAY were 96.1, 86.4, and 85.4%, respectively. WALKAWAY had a low correlation with the other two systems. Moreover, concordance rates of ABPC MIC ≥4 mg/l, which is considered as resistant, of 69 g-BLNAR isolates for the RAISUS, DP-EIKEN, and WALKAWAY systems were 68.1, 58.0, and 37.7%, respectively. Therefore, in Japan, where the BLNAR strain is isolated at a high frequency, it is necessary to understand the characteristics of the measuring systems to appropriately interpret the test results.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Haemophilus influenzae / Farmacorresistencia Bacteriana / Proteínas de Unión a las Penicilinas / Antiinfecciosos País/Región como asunto: Asia Idioma: En Revista: Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Japón
Buscar en Google
Añadir a Mi BVS
Imprimir
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Haemophilus influenzae / Farmacorresistencia Bacteriana / Proteínas de Unión a las Penicilinas / Antiinfecciosos País/Región como asunto: Asia Idioma: En Revista: Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Japón