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Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection.
Everett, Helen E; Lean, Fabian Z X; Byrne, Alexander M P; van Diemen, Pauline M; Rhodes, Shelley; James, Joe; Mollett, Benjamin; Coward, Vivien J; Skinner, Paul; Warren, Caroline J; Bewley, Kevin R; Watson, Samantha; Hurley, Shellene; Ryan, Kathryn A; Hall, Yper; Simmons, Hugh; Núñez, Alejandro; Carroll, Miles W; Brown, Ian H; Brookes, Sharon M.
Afiliación
  • Everett HE; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Lean FZX; Pathology and Animal Sciences Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Byrne AMP; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • van Diemen PM; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Rhodes S; Bacteriology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • James J; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Mollett B; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Coward VJ; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Skinner P; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Warren CJ; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Bewley KR; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
  • Watson S; Pathology and Animal Sciences Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Hurley S; Pathology and Animal Sciences Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Ryan KA; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
  • Hall Y; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
  • Simmons H; Pathology and Animal Sciences Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Núñez A; Pathology and Animal Sciences Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Carroll MW; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
  • Brown IH; Nuffield Department of Medicine, Oxford University, Oxford OX1 3SY, UK.
  • Brookes SM; Virology Department, Animal and Plant Health Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
Viruses ; 13(1)2021 Jan 15.
Article en En | MEDLINE | ID: mdl-33467732
Ferrets were experimentally inoculated with SARS-CoV-2 (severe acute respiratory syndrome (SARS)-related coronavirus 2) to assess infection dynamics and host response. During the resulting subclinical infection, viral RNA was monitored between 2 and 21 days post-inoculation (dpi), and reached a peak in the upper respiratory cavity between 4 and 6 dpi. Viral genomic sequence analysis in samples from three animals identified the Y453F nucleotide substitution relative to the inoculum. Viral RNA was also detected in environmental samples, specifically in swabs of ferret fur. Microscopy analysis revealed viral protein and RNA in upper respiratory tract tissues, notably in cells of the respiratory and olfactory mucosae of the nasal turbinates, including olfactory neuronal cells. Antibody responses to the spike and nucleoprotein were detected from 21 dpi, but virus-neutralizing activity was low. A second intranasal inoculation (re-exposure) of two ferrets after a 17-day interval did not produce re-initiation of viral RNA shedding, but did amplify the humoral response in one animal. Therefore, ferrets can be experimentally infected with SARS-CoV-2 to model human asymptomatic infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Modelos Animales de Enfermedad / Enfermedades Asintomáticas / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Modelos Animales de Enfermedad / Enfermedades Asintomáticas / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article Pais de publicación: Suiza