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Aptamer-linked in vitro expression assay for ultrasensitive detection of biomarkers.
Baek, Min-Seok; Lee, Kyung-Ho; Byun, Ju-Young; Shin, Yong-Beom; Kim, Dong-Myung.
Afiliación
  • Baek MS; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 34134, South Korea.
  • Lee KH; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 34134, South Korea.
  • Byun JY; BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea.
  • Shin YB; BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea; BioNano Health Guard Research Center (H-GUARD), Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon, 34141, South Korea.
  • Kim DM; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 34134, South Korea. Electronic address: dmkim@cnu.ac.kr.
Anal Chim Acta ; 1146: 118-123, 2021 Feb 15.
Article en En | MEDLINE | ID: mdl-33461706
Signal amplification is a key step that determines the sensitivity of molecular assays. Although studies on aptamers have mostly focused on their target-binding ability, taking advantage of the gene-coding function of nucleic acids, we demonstrate here that aptamers can be engineered into diagnostic reagents that can both recognize a target and generate highly amplified detection signals. We developed a strategy that employs a 'readable' aptamer that consists of a single-stranded aptamer and a double-stranded reporter gene. After binding to its target via the aptamer region, the reporter gene of the readable aptamer produces amplified number of signal-generating enzymes through a subsequent in vitro expression reaction. In contrast to conventional enzyme-conjugation methods, this method allows the generation of far more amplified detection signals, thereby markedly increasing the sensitivity of detection enough to analyze a target present in aM concentrations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aptámeros de Nucleótidos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aptámeros de Nucleótidos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Países Bajos