Short Preirradiation of TiO2 Nanoparticles Increases Cytotoxicity on Human Lung Coculture System.
Chem Res Toxicol
; 34(3): 733-742, 2021 03 15.
Article
en En
| MEDLINE
| ID: mdl-33459025
Anatase titanium dioxide nanoparticles (TiO2 NPs) are used in a large range of industrial applications mainly due to their photocatalytic properties. Before entering the lung, virtually all TiO2 NPs are exposed to some UV light, and lung toxicity of TiO2 NPs might be influenced by photoexcitation that is known to alter TiO2 surface properties. Although the TiO2 NPs toxicity has been extensively investigated, limited data are available regarding the toxicity of TiO2 NPs that have been pre-exposed to UV light, and their impact on humans remains unknown. In this study, five types of TiO2NPs with tailored physicochemical features were characterized and irradiated by UV for 30 min. Following irradiation, cytotoxicity, pro-inflammatory response, and oxidative stress on a human lung coculture system (A549 epithelial cells and macrophages differentiated from THP-1 cells) were assessed. The surface charge of all samples was less negative after UV irradiation of TiO2 NPs, and the average aggregate size was slightly increased. A higher cytotoxic effect was observed for preirradiated TiO2 NPs compared to nonirradiated samples. Preirradiation of TiO2 NPs had no significant impact on the pro-inflammatory response and oxidative stress as shown by a similar production of IL-8, TNF-α, and reactive oxygen species.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Titanio
/
Nanopartículas
Límite:
Humans
Idioma:
En
Revista:
Chem Res Toxicol
Asunto de la revista:
TOXICOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos