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In vivoorganized neovascularization induced by 3D bioprinted endothelial-derived extracellular vesicles.
Maiullari, Fabio; Chirivì, Maila; Costantini, Marco; Ferretti, Anna Maria; Recchia, Sandro; Maiullari, Silvia; Milan, Marika; Presutti, Dario; Pace, Valentina; Raspa, Marcello; Scavizzi, Ferdinando; Massetti, Massimo; Petrella, Lella; Fanelli, Mara; Rizzi, Marta; Fortunato, Orazio; Moretti, Fabiola; Caradonna, Eugenio; Bearzi, Claudia; Rizzi, Roberto.
Afiliación
  • Maiullari F; Gemelli Molise SpA, Campobasso, Italy.
  • Chirivì M; Istituto Nazionale Genetica Molecolare INGM 'Romeo ed Enrica Invernizzi', Milan, Italy.
  • Costantini M; Istituto Nazionale Genetica Molecolare INGM 'Romeo ed Enrica Invernizzi', Milan, Italy.
  • Ferretti AM; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Recchia S; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland.
  • Maiullari S; Institute of Chemical Sciences and Technologies "Giulio Natta", National Research Council of Italy (SCITEC-CNR), Milano, Italy.
  • Milan M; Department of Science and High Technology, University of Insubria, Como, Italy.
  • Presutti D; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Pace V; Institute of Pathology, Università Cattolica del Sacro Cuore, Rome, Italy.
  • Raspa M; Istituto Nazionale Genetica Molecolare INGM 'Romeo ed Enrica Invernizzi', Milan, Italy.
  • Scavizzi F; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Massetti M; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Petrella L; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland.
  • Fanelli M; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Rizzi M; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
  • Fortunato O; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Moretti F; Institute of Biochemistry and Cell Biology, National Research Council of Italy (IBBC-CNR), Monterotondo, Rome, Italy.
  • Caradonna E; Department of Cardiovascular Disease, IRCCS Fondazione Policlinico Universitario Agostino Gemelli, Rome, Italy.
  • Bearzi C; Laboratory of Molecular Oncology, Gemelli Molise SpA, Campobasso, Italy.
  • Rizzi R; Laboratory of Molecular Oncology, Gemelli Molise SpA, Campobasso, Italy.
Biofabrication ; 13(3)2021 04 07.
Article en En | MEDLINE | ID: mdl-33434889
Extracellular vesicles (EVs) have become a key tool in the biotechnological landscape due to their well-documented ability to mediate intercellular communication. This feature has been explored and is under constant investigation by researchers, who have demonstrated the important role of EVs in several research fields ranging from oncology to immunology and diagnostics to regenerative medicine. Unfortunately, there are still some limitations to overcome before clinical application, including the inability to confine the EVs to strategically defined sites of interest to avoid side effects. In this study, for the first time, EV application is supported by 3D bioprinting technology to develop a new strategy for applying the angiogenic cargo of human umbilical vein endothelial cell-derived EVs in regenerative medicine. EVs, derived from human endothelial cells and grown under different stressed conditions, were collected and used as bioadditives for the formulation of advanced bioinks. Afterin vivosubcutaneous implantation, we demonstrated that the bioprinted 3D structures, loaded with EVs, supported the formation of a new functional vasculaturein situ, consisting of blood-perfused microvessels recapitulating the printed pattern. The results obtained in this study favour the development of new therapeutic approaches for critical clinical conditions, such as the need for prompt revascularization of ischaemic tissues, which represent the fundamental substrate for advanced regenerative medicine applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioimpresión / Impresión Tridimensional / Vesículas Extracelulares Límite: Humans Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioimpresión / Impresión Tridimensional / Vesículas Extracelulares Límite: Humans Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido