Your browser doesn't support javascript.
loading
Detection of CRISPR-Cas9-Mediated Mutations Using a Carbon Nanotube-Modified Electrochemical Genosensor.
Kivrak, Ezgi; Pauzaite, Tekle; Copeland, Nikki A; Hardy, John G; Kara, Pinar; Firlak, Melike; Yardimci, Atike I; Yilmaz, Selahattin; Palaz, Fahreddin; Ozsoz, Mehmet.
Afiliación
  • Kivrak E; Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Izmir 35100, Turkey.
  • Pauzaite T; Department of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YQ, UK.
  • Copeland NA; Department of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YQ, UK.
  • Hardy JG; Department of Chemistry, Faculty of Science and Technology, Lancaster University, Lancaster LA1 4YB, UK.
  • Kara P; Materials Science Institute, Lancaster University, Lancaster LA1 4YB, UK.
  • Firlak M; Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Izmir 35100, Turkey.
  • Yardimci AI; Department of Chemistry, Faculty of Science and Technology, Lancaster University, Lancaster LA1 4YB, UK.
  • Yilmaz S; Department of Chemistry, Gebze Technical University, Gebze 41400, Turkey.
  • Palaz F; Department of Chemical Engineering, Izmir Institute of Technology, Izmir 35430, Turkey.
  • Ozsoz M; Department of Chemical Engineering, Izmir Institute of Technology, Izmir 35430, Turkey.
Biosensors (Basel) ; 11(1)2021 Jan 08.
Article en En | MEDLINE | ID: mdl-33429883
The CRISPR-Cas9 system has facilitated the genetic modification of various model organisms and cell lines. The outcomes of any CRISPR-Cas9 assay should be investigated to ensure/improve the precision of genome engineering. In this study, carbon nanotube-modified disposable pencil graphite electrodes (CNT/PGEs) were used to develop a label-free electrochemical nanogenosensor for the detection of point mutations generated in the genome by using the CRISPR-Cas9 system. Carbodiimide chemistry was used to immobilize the 5'-aminohexyl-linked inosine-substituted probe on the surface of the sensor. After hybridization between the target sequence and probe at the sensor surface, guanine oxidation signals were monitored using differential pulse voltammetry (DPV). Optimization of the sensitivity of the nanogenoassay resulted in a lower detection limit of 213.7 nM. The nanogenosensor was highly specific for the detection of the precisely edited DNA sequence. This method allows for a rapid and easy investigation of the products of CRISPR-based gene editing and can be further developed to an array system for multiplex detection of different-gene editing outcomes.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Nucleares / Técnicas Biosensibles / Ingeniería Genética / Mutación Puntual / Nanotubos de Carbono Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biosensors (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Nucleares / Técnicas Biosensibles / Ingeniería Genética / Mutación Puntual / Nanotubos de Carbono Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biosensors (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza